Login

Join for Free!
118238 members
table of contents table of contents

BLUF (blue light sensing using FAD) domains constitute a recently discovered class …


Biology Articles » Biophysics » Hydrogen-bond switching through a radical pair mechanism in a flavin-binding photoreceptor » Figures

Figures
- Hydrogen-bond switching through a radical pair mechanism in a flavin-binding photoreceptor

mcith_zpq80001.jpg Figure 1 X-ray structure of the R. sphaeroides AppA BLUF domain. (A and B) Close-ups of the vicinity of the FAD chromophore in two proposed orientations of the conserved Gln in dark state (A) and light state (B). The coordinates were taken from Protein Data Bank ID code 1YRX (11).

(Click image to enlarge)

mcith_zpq80002.jpg Figure 2  EADS and their corresponding lifetimes resulting from global analysis of ultrafast transient absorption experiments on the Synechocystis Slr1694 BLUF domain upon excitation at 400 nm in H2O buffer (A) and in D2O buffer (B).

(Click image to enlarge)

mcith_zpq80003.jpg Figure 3  Kinetic traces of the Synechocystis Slr1694 BLUF domain in H2O (open circles) and D2O (filled circles) upon excitation at 400 nm, with detection at 483 nm (A), 550 nm (B), 610 nm (C), and 710 nm (D). The solid lines denote the result of the target analysis. Note that the time axis is linear from −10 to 20 ps and logarithmic thereafter.

(Click image to enlarge)

mcith_zpq80004.jpg Figure 4  Target analysis and photocycle scheme of the Synechocystis Slr1694 BLUF domain. (Upper) SADS of the various molecular species in the kinetic scheme that result from the application of a target analysis to the ultrafast time-resolved data of the Synechocystis Slr1694 BLUF domain in H2O (solid lines) and D2O (dotted lines), with FAD* (blue), Q1 (black), Q2 (magenta), and the long-lived signaling state SlrRED (orange). (Lower) The photocycle scheme of Slr1694, with time constants as they follow from the target analysis and assignments of the intermediate states as described in the text.

(Click image to enlarge)

mcith_zpq80005.jpg Figure 5  The proposed reaction mechanism for photoinduced hydrogen-bond switching in the Synechocystis Slr1694 BLUF domain. See text for details.

(Click image to enlarge)

 


rating: 5.00 from 1 votes | updated on: 4 Nov 2008 | views: 10077 |

Rate article:







excellent!bad…