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In the study, the authors investigated whether HIV-1 and HSV-2 may interact …


Biology Articles » Virology » HSV-2- and HIV-1- permissive cell lines co-infected by HSV-2 and HIV-1 co-replicate HSV-2 and HIV-1 without production of HSV-2/HIV-1 pseudotype particles

Abstract
- HSV-2- and HIV-1- permissive cell lines co-infected by HSV-2 and HIV-1 co-replicate HSV-2 and HIV-1 without production of HSV-2/HIV-1 pseudotype particles

HSV-2- and HIV-1- permissive cell lines co-infected by HSV-2 and HIV-1 co-replicate HSV-2 and HIV-1 without production of HSV-2/HIV-1 pseudotype particles

Jérôme LeGoff1, Hicham Bouhlal1, Maxime Lecerf1, Christophe Klein2, Hakim Hocini1, Ali Si-Mohamed1, Martin Muggeridge3 and Laurent Bélec1

1Université Paris V, Equipe « Immunité et Biothérapie Muqueuse », Unité INSERM Internationale U743 (« Immunologie Humaine »), Centre de Recherches, Biomédicales des Cordeliers, & Laboratoire de Virologie, Hôpital Européen Georges Pompidou, Paris, France
2Service Commun d'Imagerie Cellulaire et de Cytométrie, INSERM IFR58, Centre de Recherches Biomédicales des Cordeliers, Paris, France
3Department of Microbiology and Immunology, Louisiana State, University Health Sciences Center, Shreveport, LA 71130, USA

Background

Herpes simplex virus type 2 (HSV-2) is a major cofactor of human immunodeficiency virus type 1 (HIV-1) sexual acquisition and transmission. In the present study, we investigated whether HIV-1 and HSV-2 may interact at the cellular level by forming HIV-1 hybrid virions pseudotyped with HSV-2 envelope glycoproteins, as was previously reported for HSV type 1.

Methods

We evaluated in vitro the production of HSV-2/HIV-1 pseudotypes in mononuclear CEM cells and epithelial HT29 and P4P cells. We analyzed the incorporation into the HIV-1 membrane of HSV-2 gB and gD, two major HSV-2 glycoproteins required for HSV-2 fusion with the cell membrane, in co-infected cells and in HIV-1-infected P4P cells transfected by plasmids coding for gB or gD.

Results

We show that HSV-2 and HIV-1 co-replicated in dually infected cells, and gB and gD were co-localized with gp160. However, HIV-1 particles, produced in HIV-1-infected cells expressing gB or gD after transfection or HSV-2 superinfection, did not incorporate either gB or gD in the viral membrane, and did not have the capacity to infect cells normally non-permissive for HIV-1, such as epithelial cells.

Conclusion

Our results do not support the hypothesis of HSV-2/HIV-1 pseudotype formation and involvement in the synergistic genital interactions between HIV-1 and HSV-2.

Virology Journal 2007, 4:2. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.


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