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Figure 1. The effect of different diatomite concentrations on L-dopa production by Y. lipolytica NRRL-143 (L-tyrosine consumed -
-, L-Dopa produced -×-). A total of 3.5 mg/ml L-tyrosine, 3.0 mg/ml cell biomass and varying diatomite amounts were added at the start of the biochemical reaction. The total reaction time was 50 min at 50°C.
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Figure 2.The effect of time of addition of diatomite on L-dopa production by Y. lipolytica NRRL-143 (L-tyrosine consumed --, L-Dopa produced -×-). A total of 2.0 mg/ml diatomite was added to 3.5 mg/ml L-tyrosine and 3.0 mg/ml cell biomass. The total reaction time was 50 min at 50°C.
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Figure 3.Time course of L-dopa production and L-tyrosine consumption by Y. lipolytica NRRL-143 (L-tyrosine consumed --, L-Dopa produced -×-). a. Control (3.5 mg/ml L-tyrosine and 3.0 mg/ml cell biomass) b. Test (2.0 mg/ml diatomite added 15 min after the start of reaction to 3.5 mg/ml L-tyrosine and 3.0 mg/ml cell biomass). The total reaction time was 50 min at 50°C.
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Figure 4.The effect of various levels of cell biomass (Y. lipolytica NRRL-143) on L-dopa production (L-tyrosine consumed --, L-Dopa produced -×-). a. Control (3.5 mg/ml L-tyrosine only). b. Test (3.5 mg/ml L-tyrosine and 2.0 mg/ml diatomite added 15 min after the start of reaction), The total reaction time was 50 min at 50°C.
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