Metabolic manipulation of plants to improve their nutritional quality is an important …

• Abstract
• Introduction
• Materials and Methods
• Results and Discussion
• Conclusion
• Acknowledgements
• References
• Figures
• Tables

Biology Articles » Biotechnology » Green Biotechnology » High-level tryptophan accumulation in seeds of transgenic rice and its limited effects on agronomic traits and seed metabolite profile » Tables

Tables

- High-level tryptophan accumulation in seeds of transgenic rice and its limited effects on agronomic traits and seed metabolite profile

....................................................................................................

Table 1 Spikelet fertility of OASA1D transgenic plants (R0) grown in a greenhouse

Transgene Trp content Spikelet fertility (%) Total no. of plants 20 21–40 41–60 61–80 81–100 Mean ±SD OASA1D Increased 25 19 12 7 0 30.7±21.2 63 OASA2 Normal 5 4 6 14 11 61.4±27.2 40 NBa Normal 0 1 2 3 6 76.2±19.7 12

^a Seed grown Nipponbare plants.

....................................................................................................

Table 2 Total and free Trp contents of seeds from OASA1D transgenic plants grown in a greenhouse

Five seeds of one panicle from each transgenic line were used for measurement of total Trp content and of average seed weight. Total Trp content and seed weight of Nipponbare were determined as average values for two plants. Free Trp was measured in three to five single seeds, and values presented are means ±SD. Trp content is shown as nmol Trp g^–1 dry seed weight.

^a Transgenic line harbouring GUS and hph (OASA1D lines contain OASA1D and hph).

....................................................................................................

Table 3 Nitrogen and Trp contents of seeds produced by HW lines under field or greenhouse conditions

Five seeds of Nipponbare (NB) or HW lines were analysed for nitrogen content (percentage of dry seed weight) and total Trp content (nmol g^–1 dry seed weight) in each of the indicated (in parentheses) number of experiments, and data presented are means ±SD. Values for free Trp content (nmol g^–1 dry seed weight) are presented as means ±SD for the numbers of seeds indicated in parentheses. Means followed by the same letters are not significantly different at the 5% level (Student's t test).

^a Relative value compared with Nipponbare.

....................................................................................................

Table 4 Free amino acid composition of seeds produced by field-grown HW lines

Data are expressed as nmol of amino acid g^–1 dry seed weight and are means ±SD of values from six seeds of Nipponbare (NB) or HW lines. Means followed by the same letters are not significantly different at the 5% level (Student's t test).

^a Amount of each amino acid as a percentage of total amino acids.

^b Amount of each amino acid relative to that in Nipponbare.

....................................................................................................

Table 5 Agronomic traits of HW1 and HW5 lines

Means followed by the same letters are not significantly different at the 5% level (Student's t test).

^an means repetition. The number of plants or plots for the greenhouse and field conditions, respectively. Heading date, culm length, panicle length, and the number of tillers were recorded for each of 10 or 23 plants in the greenhouse condition and for each of 15 plants per plot in the field condition.

^b Spikelet fertility was recorded for all panicles of 10 plants in 2002 and for three panicles of main culms of 60 plants in 2003.

^c Mean dry matter of harvested seeds which were air-dried (assumed moisture content of seeds was approximately 10–15%).

^d Pollen fertility was recorded for three spikelets of each of three randomly selected plants. It was estimated from microscopic analysis of the shape and color of the pollen stained with I-KI solution (1%).

^e The weight of 1000 seeds was determined from that of 20 or 100 grains in the greenhouse and field conditions, respectively.

....................................................................................................

Table 6 Germination percentage and time to seedling establishment for HW lines

The germination test was performed with 20 seeds from greenhouse-grown plants and 15 seeds from field-grown plants. Data are means of values from two experiments.

....................................................................................................