Hepatoprotective effect of root bark of Oroxylum indicum on carbon tetrachloride (CCl4) - induced hepatotoxicity in experimental animals
Maitreyi Zaveria, c and Sunita Jainb
The present study was designed to investigate the effect of Oroxylum indicum against carbon tetrachloride (CCl4)-induced hepatotoxicity in mice and rats. The hepatotoxicity was induced with the administration of 1:1(v/v) mixture of CCl4 in arachis oil at the dose of 1ml/kg intraperitoneally on day 3rd and 6th. Pretreatment with different extracts and standard silymarin (25mg/kg) was done orally from day 1 to 7. Alcoholic (300 mg/kg), petroleum ether (300 mg/kg) and n-butanol (300–100 mg/kg) extracts produced significant (p<0.05) lowering of the elevated Serum glutamic oxaloacetic transaminase (SGOT), Serum glutamic pyruvate transaminase (SGPT), alkaline phosphatase (ALP) and total bilirubin (TB) when compared with the toxic control. The increased lipid peroxide (LPO) formation in the tissues of CCl4 –treated animals was significantly inhibited by Oroxylum indicum. The observed decreased antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH), and antioxidant concentration of glutathione were nearly normalized by Oroxylum indicum treatment. Carbon tetrachloride (CCl4)-induced damage pro+duced alteration in the antioxidant status of the tissues, which was also manifested by abnormal histopathology. Pretreatment with Oroxylum indicum restored all these changes. Hence, it is suggested that root bark of Oroxylum indicum showed significant antioxidant activity, which might be in turn responsible for its hepatoprotective activity.
Key words: Oroxylum indicum; hepatoprotective; antioxidant; carbon tetrachloride; silymarin.