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A study was carried out to determine the effect of these substances …


Biology Articles » Botany » Gray mold severity and vase life of rose buds after pulsing with citric acid, salicylic acid, calcium sulfate, sucrose and silver thiosulfate » Results

Results
- Gray mold severity and vase life of rose buds after pulsing with citric acid, salicylic acid, calcium sulfate, sucrose and silver thiosulfate

Effect of the treatments on the progress of the disease

In the assay without inoculation, there was a significant difference in the AUDPC between treatments for stages I (commercial harvest stage) and II (24 h after commercial harvest stage), but not for stage III (48 h after commercial harvest stage) (Table 2). For stage I, calcium sulfate was the most effective, reducing AUDPC by 59%. The other treatments did not differ from the controls. For stage II, only calcium sulfate differed from the control, reducing the AUDPC by 61%. However, calcium sulfate did not differ from the other substances. For stage III, no differences were found among treatments. In the repetition of the assay, at stage I, only calcium sulfate was different from the treatments and the controls, reducing AUDPC by 62%. At stages II and III, only calcium sulfate differed from the controls, and reduced AUDPC by 66% and 56%, respectively, but it did not differ from the other treatments.

In the assay with artificial inoculation, for stage I, there was no difference between treatments (Table 2). For stage II, the effects of salicylic acid, sucrose, and calcium sulfate generated a smaller AUDPC than the control. For stage III, all treatments but citric acid differed from the control. In the repetition of the assay, for stage I, pulsing with salicylic acid was the only treatment that differed from the controls. For stage II, no difference between pulsing with salicylic acid and calcium sulfate was observed, but they differed from the controls and the other treatments. For stage III, salicylic acid differed from the other treatments, including the control. Overall, for both assays, values of AUDPC did not differ among harvest stages.

Effect of the treatments on maximum severity

For this variable, statistical analysis was proceeded just for the assay without inoculation (Table 3). All flowers of the assay with inoculation were rated at the highest severity for the last evaluation. In the assay without inoculation, considering stage I, the effect of calcium sulfate was statistically different from the other treatments and the control. Sucrose was also different from the control. For stage II all treatments differed from the control, and pulsing with calcium sulfate was the most effective. For stage III, pulsing with calcium sulfate and sucrose reduced maximum severity by 72% and 50%, respectively, which were different from the treatments with citric and salicylic acids. In the repetition of the assay, all treatments differed from the controls for the three stages. Again, calcium sulfate was the most effective reducing maximum severity by 78%, 78%, and 69% for stages I, II, and III, respectively.

 Effect of the treatments on vase life

In the assay without inoculation, all substances but salicylic acid increased the vase life of the flowers (Table 4). For all three stages, pulsing with calcium sulfate was the most effective. In the repetition of the assay, calcium sulfate was again the most effective and increased the flowers vase life by 32% for stage III. Overall, in the assay with inoculation, only treatment with calcium sulfate increased the vase life of the flowers. 

Effect of STS on the variables studied

In the assay without inoculation the values of AUDPC were not affected by the pulsing times to which the flowers were exposed to STS. However, for the assay with inoculation, differences were detected between pulsing times, where a quadratic tendency in reducing AUDPC was observed (Figure 1).

In considering maximum severity, differences were detected between pulsing times only in the assay without inoculation. A very similar quadratic tendency to reduction of maximum severity values was found both times the assay was conducted (Figure 2). In the assay with inoculation, all flowers received the maximum score for severity.

Treatment with STS increased the vase life of the flowers both times the assay with inoculation was conducted. Here, as the two very similar quadratic curves show, the vase life increased as pulsing time increased (Figure 3A). In the assay with inoculation, vase life increased with increased pulsing time only the first time the experiment was conducted (Figure 3B). In the repetition of the assay no differences were found among treatments.


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