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Figure 1 The predominant two variants (no/no and no/in) of VK210 type of pvcs gene observed in the 150 isolates from Kolkata, India. M is a 100 bp DNA ladder. Co and Pr and Po stand for control (nested PCR product), pre-repeat region digested by ScrF1 enzyme and post-repeat region digested by Bbs1 enzyme, respectively. The three size variants a, b and c are 740, 710 and 680 bp, respectively.
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Figure 2 Allele frequency distribution of pvcs observed in the 151 Kolkata isolates.
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Figure 3 A) Dominant alleles by Alu1 restriction enzyme digestion of the second fragment of pvmsp1 gene. B) Dominant alleles by Mnl1 restriction enzyme digestion of the second fragment of pvmsp1 gene. M is a DNA marker in 100 bp steps.
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Figure 4 The allele frequency distribution of the second fragment of pvmsp1 locus in the 151 Kolkata isolates characterized by a combination of fragment size and sequence type using RFLP.
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Figure 5 Three sizes of pvmsp3-alpha gene product in the 151 Kolkata isolates. The three sizes a, b and c represented are 1900, 1500 and 1100 bp, respectively. M is a 100 bp DNA ladder marker and M is a 1 kb DNA ladder. Double bands reflect infection with multiple genotypes.
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Figure 6 Frequency distribution of alleles at the pvmsp3-alpha loci observed in the 151 Kolkata isolates.
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Source: Malaria Journal 2006, 5:71
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