Login

Join for Free!
112189 members
table of contents table of contents

The genotyping protocols used in this study may be useful for differentiating …


Biology Articles » Parasitology » Genetic diversity of Plasmodium vivax in Kolkata, India » Figures

Figures
- Genetic diversity of Plasmodium vivax in Kolkata, India

..................................................

Figure 1 The predominant two variants (no/no and no/in) of VK210 type of pvcs gene observed in the 150 isolates from Kolkata, India. M is a 100 bp DNA ladder. Co and Pr and Po stand for control (nested PCR product), pre-repeat region digested by ScrF1 enzyme and post-repeat region digested by Bbs1 enzyme, respectively. The three size variants a, b and c are 740, 710 and 680 bp, respectively.

figure 1

..................................................

Figure 2 Allele frequency distribution of pvcs observed in the 151 Kolkata isolates.

figure 2

..................................................

Figure 3 A) Dominant alleles by Alu1 restriction enzyme digestion of the second fragment of pvmsp1 gene. B) Dominant alleles by Mnl1 restriction enzyme digestion of the second fragment of pvmsp1 gene. M is a DNA marker in 100 bp steps.

figure 3

..................................................

Figure 4 The allele frequency distribution of the second fragment of pvmsp1 locus in the 151 Kolkata isolates characterized by a combination of fragment size and sequence type using RFLP.

figure 4

..................................................

Figure 5 Three sizes of pvmsp3-alpha gene product in the 151 Kolkata isolates. The three sizes a, b and c represented are 1900, 1500 and 1100 bp, respectively. M is a 100 bp DNA ladder marker and M is a 1 kb DNA ladder. Double bands reflect infection with multiple genotypes.

figure 5

..................................................

Figure 6 Frequency distribution of alleles at the pvmsp3-alpha loci observed in the 151 Kolkata isolates.

figure 6

..................................................

Source: Malaria Journal 2006, 5:71     


rating: 3.55 from 11 votes | updated on: 20 Dec 2006 | views: 6391 |

Rate article:







excellent!bad…