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In this study, hematopoietic precursors were shown to acquire long-term multilineage hematopoietic …


Biology Articles » Methods & Techniques » Gene transfer to pre-hematopoietic and committed hematopoietic precursors in the early mouse Yolk Sac: a comparative study between in situ electroporation and retroviral transduction » Table

Table
- Gene transfer to pre-hematopoietic and committed hematopoietic precursors in the early mouse Yolk Sac: a comparative study between in situ electroporation and retroviral transduction

Table 1
In situelectroporation efficiently delivers the construct to the blood islands

Frequency of recovered hematopoietic precursors
Number of recovered hematopoietic precursors per YS
Number of experiments
Whole OrgD3-YS
1/475
10–11
4
Whole OrgD3-YS electroporated without plasmid
1/453
10
3
OrgD3-YS GFP+cells
1/150 (± 57.62)
0.56 ± 0.18 per 0.5 YS (targeted area)
5
Hematopoietic precursor frequency within whole control YS (electroporated without construct) and GFP+ cells sorted from electroporated YS-explants were determined through limiting dilution assay.

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