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In this study, hematopoietic precursors were shown to acquire long-term multilineage hematopoietic …


Biology Articles » Methods & Techniques » Gene transfer to pre-hematopoietic and committed hematopoietic precursors in the early mouse Yolk Sac: a comparative study between in situ electroporation and retroviral transduction » Conclusion

Conclusion
- Gene transfer to pre-hematopoietic and committed hematopoietic precursors in the early mouse Yolk Sac: a comparative study between in situ electroporation and retroviral transduction

In summary (Table 2), we provide and detail here two complementary methods to transfer genes in YS cells, at the earliest stages of hematopoietic development (7–8 dpc). We studied the influence of several parameters and hence determined the most convenient for either early (mesodermal/pre-hematopoietic) and late (hematopoietic) precursor transduction. These two methods can be used to either enforce (as exemplified here) or knock-down (through vectorized siRNA) the expression of potentially important genes. They are thus likely to increase our knowledge of the mechanisms giving rise to primitive hematopoiesis and to understand the molecular basis underlying its limited potency, when compared to true HSC from the AGM or adult BM.


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