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Table
- FRAP analysis of photosynthetic membranes

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Table 1. Laser light sources, dichroic mirrors and filters used to visualize photosynthetic pigments/complexes or dyes in the Nikon PCM2000 confocal microscope The dichroic mirror is used to separate the laser excitation light from the fluorescence. Its cut-off wavelength should be longer than the laser excitation laser wavelength, but shorter than the emission maximum of the fluorophore, if possible. An emission filter is used to select the fluorescence wavelengths to be observed.

Pigment/complex Organism Laser Wavelength Dichroic Emission filter Transmission

Intact phycobilisomes Cyanobacteria Red HeNe 633 nm 650 nm Schott RG665 >665 nm
Phycocyanin Cyanobacteria Red HeNe 633 nm 650 nm Interference edge filter >650 nm
Phycoerythrin Cyanobacteria Green HeNe 543 nm 565 nm Interference band-pass (605±15) nm
Photosystem II/IsiA Cyanobacteria Argon 457 nm 475 nm Schott RG665 >665 nm
Photosystem II/LHCII Plants Argon 488 nm 505 nm Schott RG665 >665 nm
GFP Any Argon 488 nm 505 nm Interference band- pass (515±15) nm
BODIPY FL C12 Any Argon 488 nm 505 nm Interference band-pass (515±15) nm

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