Available in vitro and in vivo methods for verifying protein substrates for …

• Abstract
• Background
• Results
• Discussion
• Conclusion
• Methods
• Abbreviations
• References
• Figures

Savings in time for experimentation and the higher sensitivity for detecting ³H-labeled lipid anchors recommend the TLC-scanning method with GST-tagged or HA-tagged target proteins as the method of choice for analyzing their prenylation capabilities in vitro and in vivo and, possibly, also for studying the myristoyl and palmitoyl posttranslational modifications.