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Available in vitro and in vivo methods for verifying protein substrates for …


Biology Articles » Biochemistry » Protein Biochemistry » Farnesylation or geranylgeranylation? Efficient assays for testing protein prenylation in vitro and in vivo » Conclusion

Conclusion
- Farnesylation or geranylgeranylation? Efficient assays for testing protein prenylation in vitro and in vivo

Savings in time for experimentation and the higher sensitivity for detecting 3H-labeled lipid anchors recommend the TLC-scanning method with GST-tagged or HA-tagged target proteins as the method of choice for analyzing their prenylation capabilities in vitro and in vivo and, possibly, also for studying the myristoyl and palmitoyl posttranslational modifications.


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