Login

Join for Free!
118325 members
table of contents table of contents

The authors herein report the expression and secretion of the rat CD11…


Biology Articles » Biotechnology » Red Biotechnology » Expression by Streptomyces lividans of the Rat Alpha Integrin CD11b A-Domain as a Secreted and Soluble Recombinant Protein

Abstract
- Expression by Streptomyces lividans of the Rat Alpha Integrin CD11b A-Domain as a Secreted and Soluble Recombinant Protein

Research Article

Expression by Streptomyces lividans of the Rat α Integrin CD11b A-Domain as a Secreted and Soluble Recombinant Protein

Dorra Zouari Ayadi,1 Hichem Chouayekh,1 SondaMhiri,1 Khaled Zerria,2 DahmaniM. Fathallah,2and Samir Bejar1

1Laboratory of Enzymes and Metabolites of Prokaryotes, Center of Biotechnology of Sfax, BP “K”, 3038 Sfax, Tunisia
2Molecular Biotechnology Group, Laboratory of Immunology, Institute Pasteur of Tunis, BP 74, 1002 Belv´ed`ere, Tunis, Tunisia

We already reported the use of a long synthetic signal peptide (LSSP) to secrete the Streptomyces sp. TO1 amylase by Streptomyces lividans strain. We herein report the expression and secretion of the rat CD11b A-domain using the same LSSP and S. lividans as host strain. We have used the Escherichia coli/Streptomyces shuttle vector pIJ699 for the cloning of the A-domain DNA sequence downstream of LSSP and under the control of the constitutive ermE-up promoter of Streptomyces erythraeus. Using this construct and S. lividans as a host strain, we achieved the expression of 8mg/L of soluble secreted recombinant form of the A-domain of the rat leukocyte β2 integrin CD11/CD18 alpha M subunit (CD11b). This secreted recombinant CD11b A-domain reacted with a function blocking antibody showing that this protein is properly folded and probably functional. These data support the capability of Streptomyces to produce heterologous recombinant proteins as soluble secreted form using the “LSSP” synthetic signal peptide.

Journal of Biomedicine and Biotechnology, Volume 2007, Article ID 54327, 6 pages. This is an open access article distributed under the Creative Commons Attribution License.


rating: 0.00 from 0 votes | updated on: 13 Dec 2007 | views: 4820 |

Rate article:







excellent!bad…