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Hydromedusa maximiliani is a vulnerable neotropical freshwater turtle endemic to mountainous regions …


Biology Articles » Genetics » Ecological Genetics » Estimating dispersal and gene flow in the neotropical freshwater turtle Hydromedusa maximiliani (Chelidae) by combining ecological and genetic methods » Results

Results
- Estimating dispersal and gene flow in the neotropical freshwater turtle Hydromedusa maximiliani (Chelidae) by combining ecological and genetic methods

Nine of the 80 primers used yielded band patterns that were clear and could be scored with confidence. These primers produced 27 potentially scoreable bands (range 1-6; mean 3), of which nine bands (33%) were polymorphic and produced 12 RAPD phenotypes. The estimated allele frequencies at RAPD-encoding loci from these nine polymorphic bands varied among rivers and streams, indicating that most loci were spatially structured (Table I) and endemic to some rivers and streams. Locus 2 was found only for site A (river 1), which was the river located farthest west in this drainage. Two unique alleles were found for turtles inhabiting river 4 at site B (loci 5 and 9), while one unique allele (locus 8) was found for individuals from river 2 (site B). One allele, locus 1, was the most widespread allele and was detected at all three sites, including rivers 2, 4, 7, and 8 (Table I).

The estimated FST for each locus ranged from 0.077 to 0.743 (Table II), and only one locus (locus 7) showed no significant difference among populations (p > 0.05). The overall differentiation (FST = 0.293) among populations of turtles inhabiting different rivers and streams from the sampled drainage was significant (c218 = 132.03; p < 0.0001), indicating variation among populations. The average Nm estimate was 0.60 (range = 0.09-3.00).


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