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The reproducibilty of dengue IgM and IgG ELISA was studied in serum …


Biology Articles » Biochemistry » Enzymology » Enzyme-linked immunoassay for dengue virus IgM and IgG antibodies in serum and filter paper blood » Tables

Tables
- Enzyme-linked immunoassay for dengue virus IgM and IgG antibodies in serum and filter paper blood

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Table 1
Dengue antibody detection by ELISA in serum and reconstituted filter paper blood of Vietnamese febrile patients, repeated tests and storage time of specimens.

Dengue tests in febrile patients

Upon presentation (t0)
After convalescence (t3)


IgM
IgG
IgM
IgG
Total cases tested
781
Storage time (days, median, range)
252 (57 – 751) days
228 (36 – 730)
No. positive sera (index value ≥1)
222 (28%)
673 (86%)
261 (33%)
679 (87%)
No. cases tested twice
73
74
73
73
Median storage time first test (days, range)
114 (57 – 283)
92.5 (36 – 253)
Median storage time second test (days, range)
280 (225 – 798)
259 (204 – 777)
No. positive first test
9 (12%)
59 (80%)
17 (23%)
56 (77%)





No. positive second tests
23 (32%)
63 (85%)
23 (32%)
61 (84%)
P value (Chi Square test)
No. of combined sera and blood spots on filter paper tested
161
Median storage time (days, range)
283 (139 – 795)
262 (118 – 774)
No. positive sera
46 (29%)
121 (75%)
89 (55%)
128 (80%)
No. positive filter papers
8 (5%)
97 (60%)
55 (34%)
123 (76%)
P value (Chi Square test)
Kappa value
0.191
0.391
0.567
0.549
t3: three weeks after t0.

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Table 2
Serological diagnosis of dengue, based on ELISA on serum versus ELISA on reconstituted filter paper blood.
Diagnosis based on ELISA on filter paper
Diagnosis based on ELISA on serum
Total

acute primary
acute secondary
no dengue
past dengue

Acute primary
12
1
0
0
13
Acute secondary
10
33
0
3
52
no dengue
5
2
17
13
37
past dengue
9
20
4
32
59
Total
36
56
21
48
161a)
a) Overall kappa value: 0.43, P value

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