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A chromosome engineering strategy that allows the generation of chromosomes with this …


Biology Articles » Bioengineering » Engineering translocations with delayed replication: evidence for cis control of chromosome replication timing » Table 3

Table 3
- Engineering translocations with delayed replication: evidence for cis control of chromosome replication timing

Frequencies of GCRs involving Cre-dependent translocations

 

Cell line Chr.3 Chr.6 Chr.10 Chr.13 Chr.15 Chr.16 New GCRs involving T

P175F1 N/A <0.01 <0.01 N/A N/A N/A N/A
P175F2 N/A <0.01 <0.01 N/A N/A N/A N/A
P175F3 N/A <0.01 <0.01 N/A N/A N/A N/A
P175F4 N/A <0.01 <0.01 N/A N/A N/A N/A
R175F1 N/A T T N/A N/A N/A 0.1
R175F2 N/A T T N/A N/A N/A 0.09
R175F3 N/A T T N/A N/A N/A 0.94
R175F4 N/A T T N/A N/A N/A 0.02
P186F1 <0.01 N/A N/A <0.01 N/A N/A N/A
P186F2 <0.01 N/A N/A <0.01 N/A N/A N/A
P186F3 <0.01 N/A N/A <0.01 N/A N/A N/A
P186F4 <0.01 N/A N/A <0.01 N/A N/A N/A
R186F1 T N/A N/A T N/A N/A 0.85
R186F2 T N/A N/A T N/A N/A 0.99
R186F3 T N/A N/A T N/A N/A 0.23
R186F4 T N/A N/A T N/A N/A 0.99
P244F1 N/A N/A N/A <0.01 N/A <0.01 N/A
P244F2 N/A N/A N/A <0.01 N/A <0.01 N/A
P244F3 N/A N/A N/A <0.01 N/A <0.01 N/A
P244F4 N/A N/A N/A <0.01 N/A <0.01 N/A
R244F1 N/A N/A N/A T N/A T <0.01
R244F2 N/A N/A N/A T N/A T <0.01
R244F3 N/A N/A N/A T N/A T 0.01
R244F4 N/A N/A N/A T N/A T <0.01
R244F5 N/A N/A N/A T N/A T <0.01
P268F1 N/A N/A N/A N/A <0.01 <0.01 N/A
P268F2 N/A N/A N/A N/A <0.01 <0.01 N/A
P268F3 N/A N/A N/A N/A <0.01 <0.01 N/A
P268F4 N/A N/A N/A N/A <0.01 <0.01 N/A
R268F1 N/A N/A N/A N/A T T 0.19
R268F2 N/A N/A N/A N/A T T 0.95
R268F3 N/A N/A N/A N/A T T 1.0
R268F4 N/A N/A N/A N/A T T 1.0
R268F5 N/A N/A N/A N/A T T 1.0
P268{Delta}NH1 N/A N/A N/A N/A <0.01 <0.01 N/A
P268{Delta}NH2 N/A N/A N/A N/A <0.01 <0.01 N/A
P268{Delta}NH3 N/A N/A N/A N/A <0.01 <0.01 N/A

Mitotic spreads from each clone were analyzed by FISH using WCPs which hybridized to the Cre-dependent translocations: 6 and 10 for P175/R175, 3 and 13 for P186/R186, 13 and 16 for P244/R244, and 15 and 16 for P268/R268/P268{Delta}NH. One hundred mitotic spreads were scored for each clone with each WCP. The frequencies of GCRs involving each chromosome are indicated. The frequencies of GCRs involving the Cre-dependent translocations (T) represent the product of GCRs involving both WCPs. N/A, not applicable.


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