A chromosome engineering strategy that allows the generation of chromosomes with this …

• Abstract
• Introduction
• Results
• Discussion
• MATERIALS AND METHODS
• References
• Table 1
• Table 2
• Table 3
• Figures

Frequencies of GCRs involving Cre-dependent translocations

Cell line Chr.3 Chr.6 Chr.10 Chr.13 Chr.15 Chr.16 New GCRs involving T P175F1 N/A <0.01 <0.01 N/A N/A N/A N/A P175F2 N/A <0.01 <0.01 N/A N/A N/A N/A P175F3 N/A <0.01 <0.01 N/A N/A N/A N/A P175F4 N/A <0.01 <0.01 N/A N/A N/A N/A R175F1 N/A T T N/A N/A N/A 0.1 R175F2 N/A T T N/A N/A N/A 0.09 R175F3 N/A T T N/A N/A N/A 0.94 R175F4 N/A T T N/A N/A N/A 0.02 P186F1 <0.01 N/A N/A <0.01 N/A N/A N/A P186F2 <0.01 N/A N/A <0.01 N/A N/A N/A P186F3 <0.01 N/A N/A <0.01 N/A N/A N/A P186F4 <0.01 N/A N/A <0.01 N/A N/A N/A R186F1 T N/A N/A T N/A N/A 0.85 R186F2 T N/A N/A T N/A N/A 0.99 R186F3 T N/A N/A T N/A N/A 0.23 R186F4 T N/A N/A T N/A N/A 0.99 P244F1 N/A N/A N/A <0.01 N/A <0.01 N/A P244F2 N/A N/A N/A <0.01 N/A <0.01 N/A P244F3 N/A N/A N/A <0.01 N/A <0.01 N/A P244F4 N/A N/A N/A <0.01 N/A <0.01 N/A R244F1 N/A N/A N/A T N/A T <0.01 R244F2 N/A N/A N/A T N/A T <0.01 R244F3 N/A N/A N/A T N/A T 0.01 R244F4 N/A N/A N/A T N/A T <0.01 R244F5 N/A N/A N/A T N/A T <0.01 P268F1 N/A N/A N/A N/A <0.01 <0.01 N/A P268F2 N/A N/A N/A N/A <0.01 <0.01 N/A P268F3 N/A N/A N/A N/A <0.01 <0.01 N/A P268F4 N/A N/A N/A N/A <0.01 <0.01 N/A R268F1 N/A N/A N/A N/A T T 0.19 R268F2 N/A N/A N/A N/A T T 0.95 R268F3 N/A N/A N/A N/A T T 1.0 R268F4 N/A N/A N/A N/A T T 1.0 R268F5 N/A N/A N/A N/A T T 1.0 P268NH1 N/A N/A N/A N/A <0.01 <0.01 N/A P268NH2 N/A N/A N/A N/A <0.01 <0.01 N/A P268NH3 N/A N/A N/A N/A <0.01 <0.01 N/A

Mitotic spreads from each clone were analyzed by FISH using WCPs which hybridized to the Cre-dependent translocations: 6 and 10 for P175/R175, 3 and 13 for P186/R186, 13 and 16 for P244/R244, and 15 and 16 for P268/R268/P268NH. One hundred mitotic spreads were scored for each clone with each WCP. The frequencies of GCRs involving each chromosome are indicated. The frequencies of GCRs involving the Cre-dependent translocations (T) represent the product of GCRs involving both WCPs. N/A, not applicable.