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A chromosome engineering strategy that allows the generation of chromosomes with this …


Biology Articles » Bioengineering » Engineering translocations with delayed replication: evidence for cis control of chromosome replication timing » Table 2

Table 2
- Engineering translocations with delayed replication: evidence for cis control of chromosome replication timing

Cre-mediated deletion of floxed Neo or Hyg genes in P-lines does not cause DMC

 

Cell line Frequency of DMC

P27 0 (0/50)
R27A 6% (3/50)
P27{Delta}NH-5 0 (0/100)*
P27{Delta}NH-11 0 (0/100)*
P175 0 (0/30)
R175A 19% (5/30)
P175{Delta}NH-2 0 (0/37)*
P175{Delta}NH-5 0 (0/100)*
P175{Delta}NH-12 0 (0/40)*

Metaphase spreads were analyzed for DMC after FISH with centromere-specific probes relevant to the chosen cell lines (Chr.16-cen and Chr.3-cen probes for P27 derivative lines or Chr.6-cen and Chr.10-cen probes for P175 derivative lines). The frequency of DMC for the R-lines is from the initial screen for DMC and is shown for comparison. The actual numbers of metaphase spreads scored are shown in parentheses.

*P<0.05 compared to R-line.


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