A chromosome engineering strategy that allows the generation of chromosomes with this …

• Abstract
• Introduction
• Results
• Discussion
• MATERIALS AND METHODS
• References
• Table 1
• Table 2
• Table 3
• Figures

Cre-mediated deletion of floxed Neo or Hyg genes in P-lines does not cause DMC

Cell line Frequency of DMC P27 0 (0/50) R27A 6% (3/50) P27NH-5 0 (0/100)* P27NH-11 0 (0/100)* P175 0 (0/30) R175A 19% (5/30) P175NH-2 0 (0/37)* P175NH-5 0 (0/100)* P175NH-12 0 (0/40)*

Metaphase spreads were analyzed for DMC after FISH with centromere-specific probes relevant to the chosen cell lines (Chr.16-cen and Chr.3-cen probes for P27 derivative lines or Chr.6-cen and Chr.10-cen probes for P175 derivative lines). The frequency of DMC for the R-lines is from the initial screen for DMC and is shown for comparison. The actual numbers of metaphase spreads scored are shown in parentheses.

*P<0.05 compared to R-line.