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A chromosome engineering strategy that allows the generation of chromosomes with this …


Biology Articles » Bioengineering » Engineering translocations with delayed replication: evidence for cis control of chromosome replication timing » Table 1

Table 1
- Engineering translocations with delayed replication: evidence for cis control of chromosome replication timing

Summary of karyotypic analysis, and frequency of DMC

 

Cell line % DMC P-clone Insertion sites (P-clone) Translocation (R-pool) % DMC R-pool FISH+ DMC

P276 4 der5p+[p14], 22[q11.2] t(der5p;22)(p14;q11.2) 50 +
P186 0 3[q29], 11[p15], 13[q14] t(3;13)(q29;q14) 30 +
P230 0 1[q41–42], t(3q;11q)[11q24] t(1;3q;11q)(q41–42;11q24) 25 ND
P268 0 15[q24], 16[q12.1] t(15;16)(q24;q12.1) 20 +
P175 0 6[q14–15], 10[q11.2] t(6;10)(q14–15;q11.2) 19 +
P162 ND 3[q13], 4[q34] ND 19 ND
P177 ND t(3q;11q)[11q24], 15[q13] ND 18 ND
P229 ND ND ND 13 ND
P212 ND ND ND 13 ND
P277 ND ND ND 12 ND
P280 ND ND ND 9 ND
P263 2 3[p13], 6[q26] t(3;6)(p13;q26) 6
P27 0 3[p13], 16[p13.3] t(3;16)(p13;p13.3) 6 +
P38 0 10[p11.2], 21[q22] t(10;21)(p11.2;q22) 4
37 P-clones ND ND ND 2–4 ND
P161 2 1[q44], 3[q24], i(14q)[q21] t(1;i14q)(q44;q21) 0
P244 0 13[q22], 16[q12.1] t(13;16)(q22;q12.1) 0
P248 0 3[q22], i(14q)[q32] t(3;i14q)(q22;q32) 0
29 P-clones ND ND ND 0 ND
HTD114 0.5 None N/A N/A ND

The plasmid cassette insertion sites in each P-clone were determined by FISH, using the Aprt plasmids as probes, followed by R-banding. The plasmid insertion sites are denoted by brackets and translocation breakpoints by parentheses. The identity of each translocation was determined by FISH, using WCPs as probes, and subsequently confirmed by G-banding. P27/R27 and P38/R38 were described previously [From Breger et al., (9)]. The frequency of DMC was determined for each R-pool and selected P-clones and listed as percent (%). ND, not determined; N/A, not applicable.


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