the authors compared different strategies to optimize the production of bovine β…

• Abstract
• Background
• Results and Discussion
• Conclusion
• Methods
• References
• Figures
• Tables

..................................................

Figure 1 Expression cassettes for production and secretion of staphylococcal nuclease and/or bovine β-lactoglobulin. Nuc, staphylococcal nuclease, BLG, bovine β-lactoglobulin, P_nisA, nisin-inducible lactococcal promoter; RBS, ribosome binding site of usp45 gene; SP_Usp45, signal peptide of Usp45, LEISS, secretion-enhancer propeptide LEISSTCDA.

..................................................

Figure 2 Dose-dependent nisin induction of staphylococcal nuclease production and secretion. L. casei BL23 strain was co-transformed with pSEC:Nuc and pNZ9520 plasmids. Protein extracts were analyzed by immunoblotting using rabbit polyclonal anti-Nuc antibodies as described in the Materials and Methods section. Arrows indicate positions of Nuc and its precursor form (pre-Nuc).

..................................................

Figure 3 LEISS-Nuc-BLG production and secretion by L. casei BL23 and BL23 (int:nisRK) strains. Protein extracts were analyzed by immunoblotting using a specific anti-BLG monoclonal antibody for BL23 samples and rabbit polyclonal anti-Nuc antibodies for BL23 (int:nisRK) samples, as described in the Materials and Methods section. Arrows indicate the positions of LEISS-Nuc-BLG and precursor form.

..................................................