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A new method for generating high and stable protein expressing cell lines …


Biology Articles » Biotechnology » Efficient and reproducible generation of high-expressing, stable human cell lines without need for antibiotic selection » Conclusion

Conclusion
- Efficient and reproducible generation of high-expressing, stable human cell lines without need for antibiotic selection

We have shown that cotransfection of primary amniocytes with two plasmids, one expressing transforming adenoviral E1-functions and the second plasmid expressing hAAT as a gene of interest, resulted in high hAAT-expressing cell lines. In this new human cell lines protein expression of up to 30 pg/cell/day was stable for more than 90 passages and was accomplished without antibiotic selection. Moreover, the hAAT produced in the new amniocyte cell lines was fully glycosylated and sialylated. This new method shows an efficient way of developing stable and human cell lines from a non-tumorgenic, ethically accepted and easily available cell source.

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