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This investigation reports on the growth of this bacterial species on the …


Biology Articles » Geobiology » The effect of adsorbed lipid on pyrite oxidation under biotic conditions » Figures

Figures
- The effect of adsorbed lipid on pyrite oxidation under biotic conditions

mcith_07090902f01.jpg Figure 1 The amount of oxidation, based on the solution concentration of Fe, for the various pyrite systems. The initial cell density for the bacteria was 3.3 × 107cells/ml. All the systems were initially at a pH of 2.0. A.F. denotes A. ferrooxidans.

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mcith_07090902f02.jpg Figure 2 Epifluorescent images (microbes appear as bright spots) of pyrite powder after various reaction conditions. (a) Pyrite exposed to bacteria for 10 days; (b) lipid/pyrite exposed to bacteria for 10 days; (c) a different pyrite sample exposed to bacteria for 10 days and the (d) same sample after vortexing. These images suggest that A. ferrooxidans colonize untreated pyrite surfaces and that the presence of lipids inhibits this colonization. The scale bar for all the images is 10 μm.

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mcith_07090902f03.jpg Figure 3 Cell density plots for (a) bacteria bound to lipid/pyrite, (b) bacteria in solution for lipid/pyrite as a function of time. Data is plotted for two independent experiments, denoted by exp. 1 (left y-axis) and exp. 2 (right y-axis).

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mcith_07090902f04.jpg Figure 4 Cell density plots for (a) bacteria bound to pyrite, and (b) bacteria in the contacting solution as a function of time. Data is plotted for two independent experiments, denoted by exp. 1 (left y-axis) and exp. 2 (right y-axis).

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mcith_07090902f05.jpg Figure 5 AFM images of lipid covered pyrite in two different regions of surface. (a) A 15 × 15 micron scan showing a region with a heterogeneous, but relatively high lipid coverage. (b) A 4 × 4 micron scan of a region of the pyrite that had a lower lipid coverage. Certain features are labeled to emphasize the variation of lipid island heights. Features 1, 2, 3, 4, and 5 have heights of 4, 7, 10, 15, and 20 nm, respectively.

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