table of contents 
The authors unravelled new important molecular genetic details about fish orthologous DGs
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Table 1
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DG genes details based on genomic DNA sequences available at the Ensembl databank
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gene structure exon/intron (bp)
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SPECIES
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GENE
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ID ENSEMBL
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GENOME LOCATION
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exon1
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intron1
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exon2
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mini-intron
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exon3
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total length (bp)
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precursor (aa)
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H. sapiens
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DAG1
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ENSG00000173402
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chromosome 3
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285
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19977
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2403
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no
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no
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22665
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895
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M. musculus
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DAG1
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ENSMUSG00000039952
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chromosome 9
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279
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8351
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2403
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no
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no
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11033
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893
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D. rerio
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DAG1
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ENSDARG00000016153
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chromosome 22
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321
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15186
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2280
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no
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no
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17787
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866
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T. rubripes
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DAG1a
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NEWSINFRUG00000153220
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scaffold 2682
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336
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2938
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435
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137
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> 1689
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> 5674
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> 820
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T. rubripes
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DAG1b
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NEWSSINFRUG00000136139
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scaffold 75
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366
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818
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2307
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no
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no
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3491
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890
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T. nigroviridis
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DAG1a
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GSTEN00009405001
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scaffold SCAF11228
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?
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> 606
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441
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137
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1857
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> 3041
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> 765
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T. nigroviridis
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DAG1b
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GSTENG00033210001
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scaffold SCAF15033
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369
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779
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2313
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no
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no
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3461
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893
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O. latipes
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DAG1a
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ENSORLG00000010935
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chromosome 5
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297
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22628
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459
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86
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1818
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25288
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857
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O. latipes
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DAG1b
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ENSORLG00000009878
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chromosome 7
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312
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839
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2283
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no
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no
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3434
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864
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G. aculeatus
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DAG1a
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ENSGACG00000003062
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group XVII 379.49 k
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?
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?
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462
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116
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1827
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> 2405
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> 762
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G. aculeatus
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DAG1b
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ENSGACG00000002661
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group XII 444.80 k
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312
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1026
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2301
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no
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no
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3639
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870
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D. labrax
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DAG1a
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NCBI accession code DQ149510
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?
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?
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?
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> 90
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116
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> 1783
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> 1989
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> 624
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Details relative to the newly identified partial DG sequence from D. labrax are also included.
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Table 2
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Reciprocal alignment scores of human and fish DAG1 genes obtained with ClustalW
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H. sapiens DAG1
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D. labrax DAG1a
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T. nigroviridis DAG1a
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T. rubripes DAG1a
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O. latipes DAG1a
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G. aculeatus DAG1a
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T. nigroviridis DAG1b
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T. rubripes DAG1b
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O. latipes DAG1b
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G. aculeatus DAG1b
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H. sapiens DAG1
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-
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66
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64
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55
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57
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64
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53
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53
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54
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55
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D. labrax DAG1a
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66
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-
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84
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80
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80
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90
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68
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68
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64
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69
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T. nigroviridis DAG1a
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64
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84
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-
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77
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73
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79
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63
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63
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60
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66
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T. rubripes DAG1a
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55
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80
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77
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-
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68
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74
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58
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57
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55
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57
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O. latipes DAG1a
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57
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80
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73
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68
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-
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78
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57
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57
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55
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58
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G. aculeatus DAG1a
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64
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90
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79
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74
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78
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-
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64
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63
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62
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65
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T. nigroviridis DAG1b
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53
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68
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63
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58
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57
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64
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-
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90
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75
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80
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T. rubripes DAG1b
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53
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68
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63
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57
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57
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63
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90
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-
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74
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79
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O. latipes DAG1b
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54
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64
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60
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55
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55
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62
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75
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74
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-
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75
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G. aculeatus DAG1b
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55
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69
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66
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57
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58
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65
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80
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79
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75
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-
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The DAG1a and DAG1b orthologues have been highlighted in bold.
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Table 3
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Primers used in the PCR experiments
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Fish
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Primer
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Sequence primer (5'-3')
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D. rerio
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DAG1_s
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CCAGCCTTTCATCTGTGGCAA
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D. rerio
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DAG1_as
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CTTCGCACCCTTTTGGGCAC
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D. rerio
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ACT_s
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TCTTGACCCTGAAGTACCCCATT
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D. rerio
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ACT_as
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TCCTTGATGTCGCGCACAAT
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D. labrax
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FISH_ext_s
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GGGCTTCAGCACATGAAGAT
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D. labrax
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FISH_ext_as
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CTGTAGGG(A/G)GTCATGTTCTT
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D. labrax
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ACT s
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TCCTGACCCTGAAGTACCCCA
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D. labrax
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ACT as
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TTGATGTCACGCACGATTTCC
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T. nigroviridis
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DAG1a s
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CAGACGTTCCTGTGTGAGGGG
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T. nigroviridis
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DAG1a as
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GCTTCGGAAGGTGCTGCTTC
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T. nigroviridis
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DAG1b s
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AGCTCAGCCTCTCACCTGTAGC
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T. nigroviridis
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DAG1b as
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GACTCGTTTCACTCCATGGACC
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T. nigroviridis
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ACT s
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CACCCTGAAGTATCCCATCGAA
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T. nigroviridis
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ACT as
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GTCTCTGACGATCTCTCGCTCAG
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The primers used to amplify the gene sequences from D. rerio and T. nigroviridis were designed using the sequences available in the database. The degenerate primers FISH_ext_s and FISH_ext_as, which allowed the fishing of the newly identified D. labrax sequence, were chosen exploiting two regions displaying very high homology within the aligned DG sequences from D. rerio and T. rubripes (see also Fig. 1).
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