Although the knowledge that nuclear and cytoplasmic proteins are modified with N-acetylglucosamine …

• Abstract
• Introduction
• Results
• Discussion
• Materials and Methods
• Miscellaneous
• References
• Figures

Bjorklund, S., Almouzni, G., Davidson, I., Nightingale, K.P., and Weiss, K. (1999) Global transcription regulators of eukaryotes. Cell, 96, 759–767.

Bowe, D.B., Kenney, N.J., Adereth, Y., and Maroulakou, I.G. (2002) Suppression of Neu-induced mammary tumor growth in cyclin D1 deficient mice is compensated for by cyclin E. Oncogene, 21, 291–298.

Comer, F.I. and Hart, G.W. (2001) Reciprocity between O-GlcNAc and O-phosphate on the carboxyl terminal domain of RNA polymerase II. Biochemistry, 40, 7845–7852.

Comtesse, N., Maldener, E., and Meese, E. (2001) Identification of a nuclear variant of MGEA5, a cytoplasmic hyaluronidase and a beta-N-acetylglucosaminidase. Biochem. Biophys. Res. Commun., 283, 634–640.

Gao, Y., Wells, L., Comer, F.I., Parker, G.J., and Hart, G.W. (2001) Dynamic O-glycosylation of nuclear and cytosolic proteins: cloning and characterization of a neutral, cytosolic beta-N-acetylglucosaminidase from human brain. J. Biol. Chem., 276, 9838–9845.

Glass, C.K. and Rosenfeld, M.G. (2000) The coregulator exchange in transcriptional functions of nuclear receptors. Genes Dev., 14, 121–141.

Goldberg, H.J., Whiteside, C.I., Hart, G.W., and Fantus, I.G. (2006) Posttranslational, reversible O-glycosylation is stimulated by high glucose and mediates plasminogen activator inhibitor-1 (PAI-1) gene expression and Sp1 transcriptional activity in glomerular mesangial cells. Endocrinology, 147, 222–231.

Hahn, S. (1998) The role of TAFs in RNA polymerase II transcription. Cell, 95, 579–582.

Hartweck, L.M., Scott, C.L., and Olszewski, N.E. (2002) Two O-linked N-acetylglucosamine transferase genes of Arabidopsis thaliana L. Heynh. have overlapping functions necessary for gamete and seed development. Genetics, 161, 1279–1291.

Heinzel, T., Lavinsky, R.M., Mullen, T.M., Soderstrom, M., Laherty, C.D., Torchia, J., Yang, W.M., Brard, G., Ngo, S.D., Davie, J.R., and others. (1997) A complex containing N-CoR, mSin3 and histone deacetylase mediates transcriptional repression [see comments]. Nature, 387, 43–48.

Ishizuka, T. and Lazar, M.A. (2003) The N-CoR/histone deacetylase 3 complex is required for repression by thyroid hormone receptor. Mol. Cell Biol., 23, 5122–5131.

Jackson, S.P. and Tjian, R. (1988) O-glycosylation of eukaryotic transcription factors: implications for mechanisms of transcriptional regulation. Cell, 55, 125–133

Jiang, M.S. and Hart, G.W. (1997) A subpopulation of estrogen receptors are modified by O-linked N-acetylglucosamine. J. Biol. Chem., 272, 2421–2428.

Khan, S., Abdelrahim, M., Samudio, I., and Safe, S. (2003) Estrogen receptor/Sp1 complexes are required for induction of cad gene expression by 17beta-estradiol in breast cancer cells. Endocrinology, 144, 2325–2335.

Konrad, R.J., Zhang, F., Hale, J.E., Knierman, M.D., Becker, G.W., and Kudlow, J.E. (2002) Alloxan is an inhibitor of the enzyme O-linked N-acetylglucosamine transferase. Biochem. Biophys. Res. Commun., 293, 207–212.

Kreppel, L.K., Blomberg, M.A., and Hart, G.W. (1997) Dynamic glycosylation of nuclear and cytosolic proteins. Cloning and characterization of a unique O-GlcNAc transferase with multiple tetratricopeptide repeats. J. Biol. Chem., 272, 9308–9315

Lazarus, B.D., Love, D.C., and Hanover, J.A. (2006) Recombinant O-GlcNAc transferase isoforms: identification of O-GlcNAcase, YES tyrosine kinase and Tau as isoform-specific substrates. Glycobiology [Epub ahead of print].

Li, X., Oghi, K.A., Zhang, J., Krones, A., Bush, K.T., Glass, C.K., Nigam, S.K., Aggarwal, A.K., Maas, R., Rose, D.W., and Rosenfeld, M.G. (2003) Eya protein phosphatase activity regulates Six1-Dach-Eya transcriptional effects in mammalian organogenesis. Nature, 426, 247–254.

Liu, K., Paterson, A.J., Chin, E., and Kudlow, J.E. (2000) Glucose stimulates protein modification by O-linked GlcNAc in pancreatic beta cells: linkage of O-linked GlcNAc to beta cell death. Proc. Natl. Acad. Sci. U. S. A., 97, 2820–2825.

Liu, K., Paterson, A.J., Zhang, F., McAndrew, J., Fukuchi, K., Wyss, J.M., Peng, L., Hu, Y., and Kudlow, J.E. (2004) Accumulation of protein O-GlcNAc modification inhibits proteasomes in the brain and coincides with neuronal apoptosis in brain areas with high O-GlcNAc metabolism. J. Neurochem., 89, 1044–1055.

Love, D.C., Kochran, J., Cathey, R.L., Shin, S.H., and Hanover, J.A. (2003) Mitochondrial and nucleocytoplasmic targeting of O-linked GlcNAc transferase. J. Cell Sci., 116, 647–654

McAndrew, J., Paterson, A.J., Asa, S.L., McCarthy, K.J., and Kudlow, J.E. (1995) Targeting of transforming growth factor-alpha expression to pituitary lactotrophs in transgenic mice results in selective lactotroph proliferation and adenomas. Endocrinology, 136, 4479–4488.

Moss, B., Elroy-Stein, O., Mizukami, T., Alexander, W.A., and Fuerst, T.R. (1990) Product review. New mammalian expression vectors. Nature, 348, 91–92.

Nadal, A., Diaz, M., and Valverde, M.A. (2001) The estrogen trinity: membrane, cytosolic, and nuclear effects. News Physiol. Sci., 16, 251–255

Nagy, L., Kao, H.Y., Chakravarti, D., Lin, R.J., Hassig, C.A., Ayer, D.E., Schreiber, S.L., and Evans, R.M. (1997) Nuclear receptor repression mediated by a complex containing SMRT, mSin3A, and histone deacetylase. Cell, 89, 373–380.

O’Donnell, N. (2002) Intracellular glycosylation and development. Biochim. Biophys. Acta, 1573, 336–345.

O’Donnell, N., Zachara, N.E., Hart, G.W., and Marth, J.D. (2004) Ogt-dependent X-chromosome-linked protein glycosylation is a requisite modification in somatic cell function and embryo viability. Mol. Cell Biol., 24, 1680–1690.

Perissi, V., Aggarwal, A.K., Glass, C.K., Rose, D.W., and Rosenfeld, M.G. (2004) A corepressor/coactivator exchange complex required for transcriptional activation by nuclear receptors and other regulated transcription factors. Cell, 116, 511–526.

Perissi, V. and Rosenfeld, M.G. (2005) Controlling nuclear receptors: the circular logic of cofactor cycles. Nat. Rev. Mol. Cell Biol., 6, 542–554.

Pierce, D.F. Jr., Johnson, M.D., Matsui, Y., Robinson, S.D., Gold, L.I., Purchio, A.F., Daniel, C.W., Hogan, B.L., and Moses, H.L. (1993) Inhibition of mammary duct development but not alveolar outgrowth during pregnancy in transgenic mice expressing active TGF-beta 1. Genes Dev., 7, 2308–2317

Roh, M., Paterson, A.J., Asa, S.L., Chin, E., and Kudlow, J.E. (2001) Stage-sensitive blockade of pituitary somatomammotrope development by targeted expression of a dominant negative epidermal growth factor receptor in transgenic mice. Mol. Endocrinol., 15, 600–613.

Roh, M., Paterson, A.J., Liu, K., McAndrew, J., Chin, E., and Kudlow, J.E. (2005) Proteolytic processing of TGFalpha redirects its mitogenic activity: the membrane-anchored form is autocrine, the secreted form is paracrine. Biochim. Biophys. Acta, 1743, 231–242.

Roos, M.D. and Hanover, J.A. (2000) Structure of O-linked GlcNAc transferase: mediator of glycan-dependent signaling. Biochem. Biophys. Res. Commun., 271, 275–280.

Roos, M.D., Su, K., Baker, J.R., and Kudlow, J.E. (1997) O glycosylation of an Sp1-derived peptide blocks known Sp1 protein interactions. Mol. Cell Biol., 17, 6472–6480.

Sekinger, E.A. and Gross, D.S. (2001) Silenced chromatin is permissive to activator binding and PIC recruitment. Cell, 105, 403–414.

Shang, Y., Hu, X., DiRenzo, J., Lazar, M.A., and Brown, M. (2000) Cofactor dynamics and sufficiency in estrogen receptor-regulated transcription. Cell, 103, 843–852.

Shaw, P., Freeman, J., Bovey, R., and Iggo, R. (1996) Regulation of specific DNA binding by p53: evidence for a role for O-glycosylation and charged residues at the carboxy-terminus. Oncogene, 12, 921–930.

Shin, T.H., Paterson, A.J., Grant, J.H. III, Meluch, A.A., and Kudlow, J.E. (1992) 5-Azacytidine treatment of HA-A melanoma cells induces Sp1 activity and concomitant transforming growth factor alpha expression. Mol. Cell Biol., 12, 3998–4006.

Struhl, K. (1998) Histone acetylation and transcriptional regulatory mechanisms. Genes Dev., 12, 599–606.

Toleman, C., Paterson, A.J., Whisenhunt, T.R., and Kudlow, J.E. (2004) Characterization of the histone acetyltransferase (HAT) domain of a bifunctional protein with activable O-GlcNAcase and HAT activities. J. Biol. Chem., 279, 53665–53673.

Van Tine, B.A., Dao, L.D., Wu, S.Y., Sonbuchner, T.M., Lin, B.Y., Zou, N., Chiang, C.M., Broker, T.R., and Chow, L.T. (2004) Human papillomavirus (HPV) origin-binding protein associates with mitotic spindles to enable viral DNA partitioning. Proc. Natl. Acad. Sci. U. S. A., 101, 4030–4035.

Wells, L., Vosseller, K., and Hart G.W. (2001) Glycosylation of nucleocytoplasmic proteins: signal transduction and O-GlcNAc. Science, 291, 2376–2378.

Wong, C.W. and Privalsky, M.L. (1998) Transcriptional silencing is defined by isoform- and heterodimer-specific interactions between nuclear hormone receptors and corepressors. Mol. Cell Biol., 18, 5724–5733.

Xie, W., Chow, L.T., Paterson, A.J., Chin, E., and Kudlow, J.E. (1999) Conditional expression of the ErbB2 oncogene elicits reversible hyperplasia in stratified epithelia and up-regulation of TGFalpha expression in transgenic mice. Oncogene, 18, 3593–3607.

Xie, W., Paterson, A.J., Chin, E., Nabell, L.M., and Kudlow, J.E. (1997) Targeted expression of a dominant negative epidermal growth factor receptor in the mammary gland of transgenic mice inhibits pubertal mammary duct development. Mol. Endocrinol., 11, 1766–1781.

Yang, X., Su, K., Roos, M.D., Chang, Q., Paterson, A.J., and Kudlow, J.E. (2001) O-linkage of N-acetylglucosamine to Sp1 activation domain inhibits its transcriptional capability. Proc. Natl. Acad. Sci. U. S. A., 98, 6611–6616.

Yang, X., Zhang, F., and Kudlow, J.E. (2002) Recruitment of O-GlcNAc transferase to promoters by corepressor mSin3A: coupling protein O-GlcNAcylation to transcriptional repression. Cell, 110, 69–80.

Zhang, F., Su, K., Yang, X., Bowe, D.B., Paterson, A.J., and Kudlow, J.E. (2003) O-GlcNAc modification is an endogenous inhibitor of the proteasome. Cell, 115, 715–725.