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To directly radiolabel an anti-hepatoma mAb fragment HAb18 F(ab’)2 with 99mTc …


Biology Articles » Biophysics » Medical Biophysics » Direct technetium-99m labeling of anti-hepatoma monoclonal antibody fragment: a radioimmunoconjugate for hepatocellular carcinoma imaging » Figures

Figures
- Direct technetium-99m labeling of anti-hepatoma monoclonal antibody fragment: a radioimmunoconjugate for hepatocellular carcinoma imaging

mcith_348-01.jpg Figure 1  L-cysteine standard curve for sulphydryl d etermination using Ellman reaction.

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mcith_348-02.jpg Figure 2 Effect of reduction on integrity of 99mTc-labeled HAb18 F(ab’)2 as monitored by SDS-PAGE. Vertical lanes re present molar ratios of Sn/GH to HAb18 F(ab’)2: 1, 101; 2, 201; 3, 30 1; 4, 401; 5, 501; 6, 601. (A)Coomassie brilliant blue R250 staining. Molecular weight (kD) is indicated at the left. (B) autoradiography.

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mcith_348-03.jpg Figure 3 Effect of reduction on integrity of HAb18 F(ab’)2 as monitored by SDS*PAGE. Molecular weights (kD) are indicated at the left. Vertical lanes represent molar ratios of Sn/GH to HAb18 F(ab’)2: 1, 10001; 2, 5001; 3, 501; 4, 101; 5, unreduced F(ab’)2.

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mcith_348-04.jpg Figure 4 Binding assay for the determination of the immunoreactive fraction of 99mTc-labeled HAb18 F(ab’)2.

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mcith_348-05.jpg Figure 5 Dissociation of 99mTc-labeled HAb18 F(ab’)2 with increasing molar ratio of EDTA to mAb () and L-cys to mAb ().

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mcith_348-06.jpg Figure 6 Images of nude mice bearing human hepatocel lular carcinoma with 99mTc-HAb18 F(ab’)2 at 24h.

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