Biology Articles » Biotechnology » White Biotechnology » Development of a fluorescence-based method for monitoring glucose catabolism and its potential use in a biomass hydrolysis assay » Figures

Figures

- Development of a fluorescence-based method for monitoring glucose catabolism and its potential use in a biomass hydrolysis assay

	Figure 1 Growth curves with different glucose concentrations. Growth curves of E. coli crp*-gfp grown in modified 1 × M9 minimal media containing 2, 4, or 8% glucose. All points are the mean of four replications with error bars indicating the standard error. (a) Data was fit to curves described by the Gompertz equation. R2 values: 2%, 0.85; 4%, 0.91; 8%, 0.97. (b) Each replication consisted of the fluorescence of a crp*-gfp- culture subtracted from the fluorescence of a crp*-gfp culture. Data was fit to first derivative Gompertz equation. R2 values: 2%, 0.80; 4%, 0.82; 8%, 0.82. (c) Mean fluorescence values from panel b were integrated and plotted against mean crp*-gfp absorbance values from panel a. Linear fit R2 values: 2%, 0.90; 4%, 0.93; 8%, 0.98 (n = 4). (Click image to enlarge)
	Figure 2 Sensitivity and dynamic range. E. coli strain crp*-gfp grown in modified 1 × M9 minimal media for 20 h with increasing amounts of glucose. (a) Each time point is the average absorbance of four replications of each E. coli strain. (b) Each time point is the average fluorescence of four replications of crp*-gfp cultures minus the average fluorescence of four replications of crp*-gfp- cultures. All points are the mean ± s.e. (Click image to enlarge)
	Figure 3 Response to the addition of glucose. E. coli strains crp*-gfp and crp*-gfp- grown in modified 1 × M9 minimal media containing 2% glucose for 20 h. 8% glucose was added after 20 h to the indicated cultures. (a) Each time point is the average absorbance of three replications. (b) Each time point is the average fluorescence of three replications of crp*-gfp cultures minus the average fluorescence of three replications of crp*-gfp- cultures. All points are the mean ± s.e. (Click image to enlarge)
	Figure 4 Response to the addition of chloramphenicol. E. coli strains crp*-gfp and crp*-gfp- grown in modified 1 × M9 minimal media, containing 20% d-glucose, with or without chloramphenicol. Chloramphenicol added at 13 h. (a) Each time point for hours 0 to 12 is the average absorbance of six replications. Each time point for hours 13 to 23 is the average absorbance of three replications. (b) Each time point for hours 0 to 12 is the average fluorescence of six replications of crp*-gfp cultures minus the average fluorescence of six replications of crp*-gfp- cultures. Each time point for hours 13 to 23 is the average fluorescence of three replications of crp*-gfp cultures minus the average fluorescence of three replications of crp*-gfp- cultures. All points are the mean ± s.e. (Click image to enlarge)
	Figure 5 Real-time hydrolysis of corn stover. Simultaneous saccharification and catabolism method used to analyze corn stover samples W64A × A619 and W64A × A619 bm1. (a) Absorbance measured every 2 h for 24 h. Each point is the mean ± s.e. (n = 2). (b) Fluorescence measured 2 h for 24 h. Each point is the mean ± s.e. (n = 2). (c) Mean fluorescence values integrated and plotted over time. Each point is the mean ± s.e. (n = 2). (Click image to enlarge)

 

Table 1

Go to page: 1 2 3 4 5 6 7 8 9

rating: 0.00 from 0 votes | updated on: 17 Dec 2008 | views: 4332 |

share this article | email to friends

suggest a revision
print this page
print the whole article

Rate article:

1

2

3

4

5

excellent!bad…