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In this study, the authors determined a substrate-docking site on Csk that …


Biology Articles » Biochemistry » Determination of the substrate-docking site of protein tyrosine kinase C-terminal Src kinase » Table

Table
- Determination of the substrate-docking site of protein tyrosine kinase C-terminal Src kinase


Table 1. Catalytic parameters of Csk mutants

Activity with kdSrc
Activity with polyE4Y

kcat, min-1
Km, µM
Relative kcat/Km
kcat, min-1
Km, µg/ml
Relative kcat/Km
WT 53.8 ± 17.2 5.7 ± 2.2 1 89.6 ± 5.6 95.4 ± 16.6 1
DM 6.5 ± 1.2 13.9 ± 3.6 0.05 124.1 ± 29.8 274.1 ± 6.7 0.48
QM
7.6 ± 4.2
22.9 ± 9.4
0.03
111.1 ± 27.8
322.2 ± 32.7
0.37

Relative kcat/Km = actual kcat/Km/actual kcat/Km of WT.

Source: Proc Natl Acad Sci U S A. 2003 December 9; 100(25): 14707–14712. Copyright © 2003, The National Academy of Sciences

 



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