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Bacteriocin-producing lactic acid bacteria are commonly used as natural protective cultures.

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- Detection of the pediocin gene pedA in strains from human faeces by real-time PCR and characterization of Pediococcus acidilactici UVA1

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Primers and probe used in this study
Primer/probe	Target/specificity	Sequence (5'-3')	Product size (bp)	Reference

Bak11w	16S rDNA	AGT TTG ATC MTG GCT CAG		[35]
Bak4		AGG AGG TGA TCC ARC CGC A		[36]
P1	pedA-pedB	AAA ATA TCT AAC TAA TAC TTG
P2		TAA AAA GAT ATT TGA CCA AAA	711	[23]
pedopF	pedB-pedD	GGG CGA GTT TAA CAT GCT AGA
pedopR		TGA TTA TGA ATT AAC CGT GCA	2862	This study
pedseqA	Sequencing of the pediocin operon	CTT GCT CGA TAA TGG TAA
pedseqB		CTA TCA GGT AAC TGA AAA
pedseqC		CAC GCT TTT CTG ATG CAA
pedseqD		TTC TTG ACC CCA TTA GAA		This study
pedseqL	Upstream of operon	AGC AAT TAC AGT CAA CCA TAA CCA T
pedseqH		ACA GAG CAG GAA TGT TTG CCA CAA G	1009	This study
pedseq M	Downstream of operon	ATT GTC TCC GGC ACA ATG TT
pedseqN		CAA AGT GGG GAA ACT CGA AA	1417	This study
pedA2RTF	Real-time PCR	GGC CAA TAT CAT TGG TGG TA
pedA2RTR		ATT GAT TAT GCA AGT GGT AGC C
TqM-pedA		FAM-ACT TGT GGC AAA CAT TCC TGC TCT GTT GA-TAMRA	100	This study
Mathys et al. BMC Biotechnology 2007 7:55 doi:10.1186/1472-6750-7-55

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