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A qualitative procedure of purified DNA/RNA co-extraction from complex organic matter, …


Biology Articles » Biotechnology » Red Biotechnology » Detection of aromatic catabolic gene expression in heterogeneous organic matter used for reduction of volatile organic compounds (VOC) by biofiltration » Figures

Figures
- Detection of aromatic catabolic gene expression in heterogeneous organic matter used for reduction of volatile organic compounds (VOC) by biofiltration

mcith_9197_Fig1.gif Figure 1 1.5% agarose gel showing PCR amplifications with 16S rDNA positive control primers and differential target primers. Panel (A): amplifications with prbfo–prbre (16S-rDNA) and xylfwd–xylrev (xylM) of extracted DNA from xylene Organic matter (OM) (lane 1), methyl ethyl ketone OM (lane 2), butyl acetate OM (lane 3), ethyl acetate OM (lane 4). Panel (B): image of the 16S rDNA and tol2fwd - tol2rev primers amplicons from total DNA extracted from the C18 strain as a positive control (lane 1), xylene OM (lane 2), methyl ethyl ketone OM (lane 3), butyl acetate OM (lane 4), ethyl acetate OM (lane 5)

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mcith_9197_Fig2.gif Figure 2 1.5% agarose gel showing RT-PCR amplifications with 16S rDNA positive control primers (prbfo–prbre) and xylfwd–xylrev (xylM) target primers starting from purified RNA. Panel (A): RT-PCR of extracted and purified RNA from xylene organic matter (OM) (lane 1), methyl ethyl ketone OM (lane 2), ethyl acetate OM (lane 3). Panel (B): RT-PCR reaction controls of the previous experiment. Image of the 16S rDNA and xylM PCR amplifications where reverse transcriptase enzymes were omitted and starting from purified RNA extracted from xylene O.M. (lane 2), methyl ethyl ketone OM (lane 3), ethyl acetate OM (lane 4); previous PCR product where template was omitted is shown in lane 1. In all samples the primers utilized to amplify the two genes are visible

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