essentially on sample pre-treatment procedures, this paper describes a
protocol for co-extraction and PCR analysis of purified DNA/RNA from
complex organic matter matrices. It could be of great practical
interest in biofiltration technology, in which the high aromatic
concentration in exhaust gas reduces biological process yields.
Management of biological processes is frequently controlled just by
monitoring physical–chemical parameters, with biological parameters
only being estimated as later confirmation. An improvement of the
qualitative and quantitative procedures for monitoring microbial
activities could thus have direct real time applications to help in the
understanding of the biodynamic requirements and maintain the right
growth conditions to maximize the elimination capacity of each
polluting compound. This is particularly important to track the
effectiveness of specific inoculation in a heterogeneous microbial
For these reasons, this work, together with other biotechnological strategies, such as the use of monoclonal antibodies raised
against catabolic enzymes (Civilini et al. 2000),
is suitable for studying microbial community activities and of help in
setting up a standardized procedure for bioremediation.
This work was supported by Friuli Venezia Giulia Council (L.R. FVG 26.2.2001 n.4, Art. 7, c.45 and L.R. FVG 3/98, Art.16 –
2002) and CRABO group grants.