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A qualitative procedure of purified DNA/RNA co-extraction from complex organic matter, …


Biology Articles » Biotechnology » Red Biotechnology » Detection of aromatic catabolic gene expression in heterogeneous organic matter used for reduction of volatile organic compounds (VOC) by biofiltration » Conclusions

Conclusions
- Detection of aromatic catabolic gene expression in heterogeneous organic matter used for reduction of volatile organic compounds (VOC) by biofiltration

Conclusions

Based essentially on sample pre-treatment procedures, this paper describes a protocol for co-extraction and PCR analysis of purified DNA/RNA from complex organic matter matrices. It could be of great practical interest in biofiltration technology, in which the high aromatic concentration in exhaust gas reduces biological process yields. Management of biological processes is frequently controlled just by monitoring physical–chemical parameters, with biological parameters only being estimated as later confirmation. An improvement of the qualitative and quantitative procedures for monitoring microbial activities could thus have direct real time applications to help in the understanding of the biodynamic requirements and maintain the right growth conditions to maximize the elimination capacity of each polluting compound. This is particularly important to track the effectiveness of specific inoculation in a heterogeneous microbial community.

For these reasons, this work, together with other biotechnological strategies, such as the use of monoclonal antibodies raised against catabolic enzymes (Civilini et al. 2000), is suitable for studying microbial community activities and of help in setting up a standardized procedure for bioremediation.

 

Acknowledgements  
 
This work was supported by Friuli Venezia Giulia Council (L.R. FVG 26.2.2001 n.4, Art. 7, c.45 and L.R. FVG 3/98, Art.16 – 2002) and CRABO group grants.

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