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P. avium, a pioneer tree species that colonizes early forest successional stages, …

Biology Articles » Ecology » Detecting local establishment strategies of wild cherry (Prunus avium L.) » Tables

Tables
- Detecting local establishment strategies of wild cherry (Prunus avium L.)

 

Table 1
Characteristics of the analysed microsatellite loci




No. of alleles
 No. of effective alleles
Locus code
 Repeat motif
 Annealing temperature
 Length of alleles (bp)
 Ror
 Wib
 Ror
 Wib
UDP96-005*
 (AC) & (TC)
 61°C
 110–156
 5
 5
 3.47
 2.59
UDP98-021*
 (TG) & (AG)
 58°C
 100–114
 3
 4
 2.01
 1.51
UDP98-410*
 (GA)
 52°C
 116–138
 6
 5
 2.56
 3.85
UDP98-412*
 (AG)
 60°C
 87–129
 7
 7
 3.90
 4.88
BPPCT 034**
 (GA)
 56°C
 222–260
 7
 7
 4.02
 4.57
BPPCT 040**
 (GA)
 56°C
 116–142
 5
 5
 2.48
 3.89


gene pool diversity
 2.88
 3.00


hypothetical gametic multilocus diversity
 694.20
 1 306.24
Microsatellite locus code according to [11]* and [12]**, the optimized annealing temperature, the range of size variation in basepairs (length of alleles), the number of alleles found at each locus, the effective number of alleles v2 according to [26] and [24, p. 324]; the gene pool diversity and the hypothetical gametic multilocus diversity vgam; Ror and Wib are the acronyms for the two analysed P. avium stands in Roringen and Wibbecke

 

Table 2
Allele frequencies detected at the six microsatellite loci in the two P. avium stands

Allele frequencies
Allele frequencies
Locus code/Alleles
 Ror
 Wib
 Locus code/Alleles
 Ror
 Wib
UDP96-005

UDP98-021

1
 0.036
 0.237
 1
 0.661
 0.795
2
 0.393
 0.160
 2
 0.000
 0.032
3
 0.286
 0.019
 3
 0.205
 0.167
5
 0.071
 0.019
 5
 0.134
 0.006
6
 0.214
 0.564








UDP98-410

UDP98-412

2
 0.214
 0.308
 1
 0.134
 0.071
4
 0.571
 0.237
 2
 0.009
 0.019
5
 0.062
 0.288
 3
 0.045
 0.141
6
 0.009
 0.000
 5
 0.232
 0.224
7
 0.116
 0.006
 6
 0.384
 0.321
8
 0.027
 0.160
 7
 0.009
 0.083



8
 0.187
 0.141






BPPCT 034

BPPCT 040

1
 0.018
 0.064
 1
 0.045
 0.090
2
 0.375
 0.321
 2
 0.143
 0.276
3
 0.134
 0.167
 3
 0.571
 0.064
4
 0.009
 0.250
 4
 0.232
 0.231
6
 0.071
 0.026
 7
 0.009
 0.340
7
 0.134
 0.141


8
 0.259
 0.032


All individuals of Roringen (Ror) and Wibbecke (Wib) are included in the calculations, also individuals with identical multilocus genotype

 

 

Table 3
Testing of identical multilocus genotypes for clonal propagation based on the absence of gene associations
Multilocus-Genotype (MLG)
 No. of individuals with identical MLG
 CnN
 C2N
 detected type one error marker type (locus)
Ror1
 3
 1.3793· 10-10
 2,2898· 10-07
 SSR (UDP96-001; UDP98-411)
Ror2
 3
 2.2205· 10-15
 2,4674· 10-11
Ror3
 2
 3.8747· 10-07
 3,8747· 10-07
Ror4
 4
 5.0660· 10-13
 1,8033· 10-06
Wib1
 8
 3,8858· 10-15
 3,8320· 10-06
 isozymes (pgm, idh, 6-pgdh, skdh, aco)
Wib2
 2
 8,4748· 10-11
 8,4748· 10-11
Wib3
 7
 2.6645· 10-15
 1,9343· 10-07
 isozymes (6-pgdh, pgm, skdh)
Wib4
 6
 0
 3,2969· 10-07
 isozyme (idh)
Wib5
 3
 5.4934· 10-13
 1,1209· 10-08
Wib6
 7
 0
 7,9074· 10-06
 isozymes (idh, skdh)
Wib7
 14
 4.3299· 10-15
 1,1990· 10-08
Wib8
 3
 1.8541· 10-14
 1,1904· 10-09
Wib9
 2
 1.6718· 10-11
 1,6718· 10-11
Wib10
 2
 6,8013· 10-08
 6,8013· 10-08
Wib11
 3
 0
 3,1075· 10-13
Wib12
 5
 0
 8,8324· 10-12
Wib13
 3
 3.1086· 10-15
 1,5363· 10-10
Multilocus genotypes (MLG) represented by at least two individuals in the P. avium stands Roringen (Ror1 to Ror4) and Wibbecke (Wib1 to Wib13), the total number of individuals with the determined identical MLG, the statistical values CnN and C2N for testing clonal vs. sexual reproduction and the marker type that detected a Type one error

 

 

Table 4
Results of the Type one error analysis
Multilocus-Genotype (MLG)
 (g)
 ω (g)
 Ar(g) for C2N = 0.05
 C2Nfor Ar(g) = 0,0275
Ror1
 1.2196· 10-5
 0.232
 0.0275
 /
Ror2
 1.2658· 10-7
 0.054
 /
 0.0032
Ror3
 1.5866· 10-5
 0.090
 /
 0.0087
Ror4
 3.4244· 10-5
 0.268
 /
 0.0649
Wib1
 3.5754· 10-5
 0.282
 0.0162
 /
Wib2
 1.6788· 10-7
 0.071
 /
 0.0104
Wib3
 8.0274· 10-6
 0.282
 0.0162
 /
Wib4
 1.0481· 10-5
 0.166
 0.0275
 /
Wib5
 1.9321· 10-6
 0.038
 /
 0.0031
Wib6
 5.1381· 10-5
 0.282
 0.0161
 /
Wib7
 1.9983· 10-6
 0.224
 /
 0.0830
Wib8
 6.2936· 10-7
 0.052
 /
 0.0057
Wib9
 7.4621· 10-7
 0.083
 /
 0.0139
Wib10
 4.7596· 10-6
 0.282
 /
 0.1231
Wib11
 1.0203· 10-8
 0.038
 /
 0.0031
Wib12
 5.4225· 10-8
 0.064
 /
 0.0085
Wib13
 2.2618· 10-7
 0.038
 /
 0.0031
(1) the expected frequency (g) of the multilocus genotypes (MLG) in Table 3 under the assumption of random association of the alleles; (2) the least upper bound ω (g) of the frequency of the MLG set by the population frequencies of the alleles that are represented in this MLG; (3) the value of Ar(g) for which C2N = ε = 0.05 (/denotes the absence of a Type one error); (4) the value of C2N for the relative gene association Ar(g) = 0.0275 that would have avoided all of the observed Type one errors (values in bold exceed significance level ε = 0.05)

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