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The aim of this article is to present the decalcification process dynamic …

Home » Biology Articles » Anatomy & Physiology » Anatomy, Animal » Decalcification Dynamic of Dog Mineralized Tissue by Microwaves » Results

- Decalcification Dynamic of Dog Mineralized Tissue by Microwaves

The samples exposed to microwave promoted an acceleration of the decalcification process using nitric acid and EDTA as decalcified agent (Tables I and II). Using the traditional method, it was necessary 65 days in nitric acid solution, meanwhile the irradiated tooth needs only 15 days (Tables I and II), both of them with the same softly conditions that is necessary for the histological processing. The Warshwsky solution presented a slowly action being that necessary 150 days soaked in solution and 35 days when irradiated.

The morphological preservation using these two decalcified agents was similar, in exception of a little staining modification by EDTA, where the Trichrome Masson had its acidophihc staining increased. Thus, in the figures A and D, EDTA and nitric acid, respectively, showed the dentin/pulp relationship with preservation of the dentinal tubules, odontoblast and pulp connective tissue. In the figures B and C, nitric acid and EDTA, respectively, show the preservation of the mineralized tissue (cement and alveolar bone) and soft tissues (connective and periodontal tissue) at the root region. In the same region, it was photographed with polarized light (Fig. E) showing in the decalcified sample by EDTA the mainly fibers of the periodontium, placed between the cement (above) and fascicular bone (under). The polarized light permitted a better visualization of the dentin tissue, acellular cement, alveolar bone and periodontium fibers (Fig. F).

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