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The aim of this article is to present the decalcification process dynamic …

Home » Biology Articles » Anatomy & Physiology » Anatomy, Animal » Decalcification Dynamic of Dog Mineralized Tissue by Microwaves » Introduction

- Decalcification Dynamic of Dog Mineralized Tissue by Microwaves

Considering histological preparations of the mineralized biological samples, the decalcification process has an important role, because the preservation of tissue structures and its relationship are dependent of the quality and speed of the demineralization process occur (Baird et al, 1967; Hornbeck et al., 1986; Clayden, 1952; Efeoglu et al, 2006). Lillie et al, in 1951, considered that the ideal method for the mineralized tissues study being that remove the more quantity of calcium salts. With this objective, most methods and decalcified agents are described in the literature: simultaneous decalcification with posterior fixation by Bouin solution, an example, or using acids (nitric, formic, sulphurous) and its combination, as well as in different conditions of temperature and concentration (Engelbreth-Holm & Plum, 1951; Clayden; Goncalves& Oliverio, 1965). Substances capable to attach with specific metals (chelation agents) as ethylenediamine tetra acetate acid (EDTA) have also been used as decalcified agents. For teeth decalcification, Warshawsky & Moore, 1967 used EDTA isotonic dissolved in phosphate solution, neutral pH. This solution was calculated for the pH maintenance for a few weeks, promoting not only a good decalcification but also performing a better morphologic preservation of the samples.

The microwave irradiation in domestic oven has been used in diagnosis laboratories, when it was performed the tissue processing with notable time reduction. It is currently used for helping the tissue fixation, accelerating this process and preserving the morphologic and antigenic tissue characteristics (Cunningham et al., 2001; Lunardi & Britto-Garcia, 1996; Login et al, 1997; Roncarolieia et al., 1991; Ng & Nge et al., 1992).

Aiming to establish a fast and efficient teeth and other mineralized tissue decalcification, this research shows the results when it was compared two decalcified agents, nitric acid 5% and Washawsky solution (Washawsky & Moore) to 8,5% (EDTA pH 7.4) in two decalcified methods (soaked with shake up process and soaked followed by irradiation in microwave oven) in mandibular teeth dogs.

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