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The proteins identified by de novo amino acid sequencing using MALDI-TOF-TOF
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#
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Precursor ion (m/z)
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De novo peptide sequence
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Homology search peptide sequence [PEAKS(◆)/SPIDER(●)]
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Score %(◆) arbitrary(●)
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Sequence assigned to protein (Species)
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1
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1042.5265
1667.8766
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LVTFYEDR
VPAFLSAAEVEEHLR
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LVTFYEDR(◆)
VPAFLSAAEVEEHLR(◆)
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97.81(◆)
99(◆)
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Crystallin, mu (Homo Sapiens)
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2
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1631.7706
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FQNIDFAEEVYTR
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FQNIDFAEEVYTR(◆)
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99(◆)
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Guanine aminohydrolase(Homo Sapiens)
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3
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1303.7262
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VLTPELYAELR
GTGGVDTAAVGGVFDVSNADR
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VLTPELYAELR(◆)
GTGGVDTAAVGGVFDVSNADR (●)
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99(◆)
12.3(●)
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Brain creatine kinase (Homo Sapiens)
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4
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1516.6975
1954.0564
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[RT]YDESGPS[L]VHR
VAPEEHPVLLTEAPLNPK
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[QE]YDESGPS[I]VHR(●)
VAPEEHPVLLTEAPLNPK(●)
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7.6(●)
11.2(●)
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Actin beta (Homo Sapiens)
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5
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1967.8951
1648.6646
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[RS]A[L]QAAHDAVAQEGQCR
[N]GHLYELDGR
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[NE]A[I]QAAHDAVAQEGQCR(●)
[D]GHLYELDGR(●)
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10.0(●)
5.8(●)
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Ubiquitin carboxyl-terminal esterase L1 (Homo Sapiens)
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6
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1211.6733
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Q [L]TVNDL[VP]GR
EGGLGPLNIPLLADVTR
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Q[I]TVNDL[PV]GR(●)
EGGLGPLNIPLLADVTR(◆)
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5.6(●)
95.97(◆)
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Peroxiredoxin 2 isoform b (Homo Sapiens)
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7
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1684.9053
2560.2588
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AAVEEGIVLGGGCALLR
LVQDVANNTNEEAGDGTTTATVLAR
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AAVEEGIVLGGGCALLR(◆)
LVQDVANNTNEEAGDGTTTATVLAR(◆)
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98.28(◆)
98.49(◆)
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Heat shock 60 kD protein 1 (Homo Sapiens)
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8
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1987.0314
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AIAELGIYPAVDPLDSTSR
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AIAELGIYPAVDPLDSTSR(●)
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11.2(●)
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ATP synthase beta chain, mitochondrial precursor (Homo Sapiens)
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9
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1917.9341
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FQDGDLTLYQSNTFLR
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FQDGDLTLYQSNTFLR(◆)
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96.24(◆)
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GST pi enzyme (Macaca mulatta)
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10
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1256.55
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NCSETQYERK
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NCSETQYERK(◆)
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99.1(◆)
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14-3-3 gamma protein (Homo Sapiens)
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11
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1253.6162
1981.9982
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FEELNADLFR
TVTNAVVTVPAYFNDSQR
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FEELNADLFR(◆)
TVTNAVVTVPAYFNDSQR(◆)
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97.95(◆)
99.49(◆)
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Heat shock 70 kDa protein 8 isoform 1(Homo Sapiens)
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12
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1917.9427
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SIQEIQELDKDDESLR
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SIQEIQELDKDDESLR(◆)
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99.17(◆)
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Rho GDI alpha (Homo Sapiens)
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13
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1337.7222
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PPYTVVYFPVR
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PPYTVVYFPVR(◆)
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98.1(◆)
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Glutathione S-transferase
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Tannu et al. BMC Genomics 2007 8:270 doi:10.1186/1471-2164-8-270
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