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The current study validates a robust method to confidently characterize the proteins …


Biology Articles » Zoology » Primatology » De novo protein sequence analysis of Macaca mulatta » Table

Table
- De novo protein sequence analysis of Macaca mulatta

The proteins identified by de novo amino acid sequencing using MALDI-TOF-TOF

#
Precursor ion (m/z)
De novo peptide sequence
Homology search peptide sequence [PEAKS(◆)/SPIDER(●)]
Score %(◆) arbitrary(●)
Sequence assigned to protein (Species)
1
1042.5265
1667.8766
LVTFYEDR
VPAFLSAAEVEEHLR
LVTFYEDR(◆)
VPAFLSAAEVEEHLR(◆)
97.81(◆)
99(◆)
Crystallin, mu (Homo Sapiens)
2
1631.7706
FQNIDFAEEVYTR
FQNIDFAEEVYTR(◆)
99(◆)
Guanine aminohydrolase(Homo Sapiens)
3
1303.7262
VLTPELYAELR
GTGGVDTAAVGGVFDVSNADR
VLTPELYAELR(◆)
GTGGVDTAAVGGVFDVSNADR (●)
99(◆)
12.3(●)
Brain creatine kinase (Homo Sapiens)
4
1516.6975
1954.0564
[RT]YDESGPS[L]VHR
VAPEEHPVLLTEAPLNPK
[QE]YDESGPS[I]VHR(●)
VAPEEHPVLLTEAPLNPK(●)
7.6(●)
11.2(●)
Actin beta (Homo Sapiens)
5
1967.8951
1648.6646
[RS]A[L]QAAHDAVAQEGQCR
[N]GHLYELDGR
[NE]A[I]QAAHDAVAQEGQCR(●)
[D]GHLYELDGR(●)
10.0(●)
5.8(●)
Ubiquitin carboxyl-terminal esterase L1 (Homo Sapiens)
6
1211.6733
Q [L]TVNDL[VP]GR
EGGLGPLNIPLLADVTR
Q[I]TVNDL[PV]GR(●)
EGGLGPLNIPLLADVTR(◆)
5.6(●)
95.97(◆)
Peroxiredoxin 2 isoform b (Homo Sapiens)
7
1684.9053
2560.2588
AAVEEGIVLGGGCALLR
LVQDVANNTNEEAGDGTTTATVLAR
AAVEEGIVLGGGCALLR(◆)
LVQDVANNTNEEAGDGTTTATVLAR(◆)
98.28(◆)
98.49(◆)
Heat shock 60 kD protein 1 (Homo Sapiens)
8
1987.0314
AIAELGIYPAVDPLDSTSR
AIAELGIYPAVDPLDSTSR(●)
11.2(●)
ATP synthase beta chain, mitochondrial precursor (Homo Sapiens)
9
1917.9341
FQDGDLTLYQSNTFLR
FQDGDLTLYQSNTFLR(◆)
96.24(◆)
GST pi enzyme (Macaca mulatta)
10
1256.55
NCSETQYERK
NCSETQYERK(◆)
99.1(◆)
14-3-3 gamma protein (Homo Sapiens)
11
1253.6162
1981.9982
FEELNADLFR
TVTNAVVTVPAYFNDSQR
FEELNADLFR(◆)
TVTNAVVTVPAYFNDSQR(◆)
97.95(◆)
99.49(◆)
Heat shock 70 kDa protein 8 isoform 1(Homo Sapiens)
12
1917.9427
SIQEIQELDKDDESLR
SIQEIQELDKDDESLR(◆)
99.17(◆)
Rho GDI alpha (Homo Sapiens)
13
1337.7222
PPYTVVYFPVR
PPYTVVYFPVR(◆)
98.1(◆)
Glutathione S-transferase

Tannu et al. BMC Genomics 2007 8:270   doi:10.1186/1471-2164-8-270


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