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In this study, we used culture and culture-independent methods to determine the …


Biology Articles » Microbiology » Culture-independent analysis of bacterial diversity in a child-care facility » Tables

Tables
- Culture-independent analysis of bacterial diversity in a child-care facility

Table 1
Unique morphologies present in culture results, and their identification through sequencing
Organism
Sequence Length1
% Identity2
GenBank Accesssion
Morphology
Acinetobacter sp.
741
98
EF409307
Acinet. 1
Bacillus licheniformis
720
99
EF409308
Bacillus M1
Bacillus megaterium
739
99
EF409309
Bacillus M2
Bacillus sp.
498
97
EF409310
Bacillus M3
Bacillus sp.
754
100
EF409311
Bacillus M4
Bacillus sp.
724
99
EF409312
Bacillus M 5 (Nutrient)
Bacillus sp.
748
99
EF409313
Bacillus M6
Bacillus sp.
730
100
EF409314
Bacillus M7
Bacillus sp.
664
99
EF409315
Bacillus M8
Bacillus sp.
741
89
EF409316
Bacillus M9
Bacillus sp.
677
98
EF409317
Bacillus M10
Bacillus sp.
721
99
EF409318
Bacillus M11
Bacillus subtilis
759
99
EF409319
Bacillus M12
Bacillus subtilis
759
100
EF409320
Bacillus M5a
Bacillus subtilis
736
99
EF409321
Bacillus M13
Bacillus subtilis
722
99
EF409322
Bacillus M14
Bacillus subtilis
718
99
EF409323
Bacillus M15
Brevibacillus sp.
711
99
EF409324
Brevi. M1a
Brevibacterium sp.
695
99
EF409325
Brevi. M1b
Enterococcus faecalis
764
99
EF409326
Enterococ. M1
Exiguobacterium sp.
767
99
EF409327
Exiguobac. M1
Microbacterium aurum
289
99
EF409328
Microbac. M1a
Microbacterium esteraromaticum
327
98
EF409329
Microbac. M1b
Moraxella osloensis
750
98
EF409330
Morax. M1
Pseudomonas stutzeri
682
99
EF409331
Pseudom. M1
Staphylococcus aureus
752
99
EF409332
Staph. M1
Staphylococcus aureus
727
100
EF409333
Staph. M2
Staphylococcus epidermidis
721
100
EF409334
Staph. M3
Staphylococcus haemolyticus
703
99
EF409335
Staph. M4
1 Length of sequence used in BLAST search
2 Based on BLAST alignment
3 Abbreviations for each morphology type; cross-referenced in Table 2
Table 2
Culture results for environmental swab samples
Date
Plate type
Total Plates
% Growth1
Organisms detected2
30-Sep-2005
Nutrient
6
66.7
N/A

Blood
4
75.0
N/A
6-Oct-2005
Nutrient
12
0.0
N/A

Blood
12
33.3
N/A
14-Oct-2005
Nutrient
14
16.7
N/A

Blood
0
0.0
N/A
21-Oct-2005
Nutrient
24
79.2
Bacillus sp (Bacillus M7)

Blood
0
0.0
Bacillus sp. (Bacillus M2, M11)
28-Oct-2005
Nutrient
10
80.0
N/A

Blood
6
83.3
N/A
3-Nov-2005
Nutrient
13
84.6
Bacillus sp. (Bacillus M7)

Blood
12
83.3
Bacillus sp. (Bacillus M11)
15-Nov-2005
Nutrient
22
81.8
Bacillus sp. (Bacillus M7)

Blood
16
56.3
Bacillus sp. (Bacillus M3, M11, 14)
18-Jan-2006
Nutrient
16
56.3
N/A

Blood
0
0.0
N/A
25-Jan-2006
Nutrient
16
68.8
N/A

Blood
16
75.0
N/A
3-Feb-2006
Nutrient
12
75.0
Bacillus sp. (Bacillus M2, M7, M10), Staphylococcus sp. (Staph. M1)

Blood
16
81.3
Bacillus sp. (Bacillus M1, M2, M3, M14), Staphylococcus sp. (Staph. M2), Moraxella sp. (Morax. M1), Brevibacterium sp. (Brevi. M1b), Brevibacillus sp. (Brevi. M1a)
15-Feb-2006
Nutrient
10
90.0
Bacillus sp. (Bacillus M10), Staphylococcus sp. (Staph. M3)

Blood
10
80.0
Bacillus sp. (Bacillus M1), Microbacterium sp. (Microbac. M1)
1-Mar-2006
Nutrient
10
70.0
Pseudomonas sp. (Pseudo. M1), Bacillus sp. (Bacillus M1, M5, M7), Staphylococcus sp. (Staph M3)

Blood
10
60.0
Bacillus sp. (Bacillus M1, M5, M9, M11), Staphylococcus sp. (Staph. M2)
8-Mar-2006
Nutrient
20
85.0
Bacillus sp. (Bacillus M5, M7), Staphylococcus sp. (Staph. M3), Enterococcus sp. (Enterococ. M1)

Blood
20
90.0
Bacillus sp. (Bacillus M1, M11), Staphylococcus sp. (Staph. M2), Moraxella sp. (Morax. M1)
29-Mar-2006
Nutrient
16
50.0
Bacillus sp. (Bacillus M5, M7), Staphylococcus sp. (M1), Enterococcus sp. (Enterococ. M1)

Blood
16
50.0
Bacillus sp. (Bacillus M2, M5a, M11, M13), Staphylococcus sp. (M2)
5-Apr-2006
Nutrient
12
41.7
Bacillus sp. (Bacillus M7, M12), Staphylococcus sp. (Staph. M1, M3), Acinetobacter sp. (Acinet. M1)

Blood
12
58.3
Bacillus sp. (Bacillus M2, M8, M11, M14), Staphylococcus sp. (M2, M3)
12-Apr-2006
Nutrient
16
37.5
Bacillus sp. (Bacillus M7, M12), Brevibacterium sp. (Brevi. M1b), Pseudomonas sp. (Pseudo. M1), Exiguobacterium sp. (Exiguobac. M1)

Blood
16
43.8
Bacillus sp. (Bacillus M1, M2, M8, M11), Staphylococcus sp. (Staph. M2), Brevibacterium sp. (Brevi. M1a), Exiguobacterium sp. (Exiguobac. M1)
19-Apr-2006
Nutrient
16
43.8
Bacillus sp. (Bacillus M10, M12), Acinetobacter sp. (Acinet. M1), Enterococcus sp. (Enterococ. M1)

Blood
16
81.3
Bacillus sp. (Bacillus M1, 2, M15), Brevibacterium sp. (Brevi. M1b), Microbacterium sp. (Microbac. M1a)
1 Percentage of plates with microbial growth
2 Observed morphologies (see Table 1); N/A = Not Available for that sampling day.

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