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In this study, we used culture and culture-independent methods to determine the …


Biology Articles » Microbiology » Culture-independent analysis of bacterial diversity in a child-care facility » Figures

Figures
- Culture-independent analysis of bacterial diversity in a child-care facility

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Figure 1 Results of Bayesian phylogenetic analysis of Pseudomonas-related 16S rRNA gene sequences representing the most common clones found in PCR-amplified libraries from swabs samples of toys and surfaces. Cloned sequences are indicated by "CCTR" (Children's Center Toddler Room) or "CCIR" (Children's Center Infant Room) prefixes followed by the date of sampling and whether the sequence was obtained from a toy (T) or a surface (S). The phylogeny includes sequences of closely related cultured and uncultured organisms. GenBank accession numbers are presented next to the names. The values above the branches indicate the Bayesian posterior probabilities (above 0.5) under the specified model of evolution for each node (see Methods for details). Maximum Parsimony and Maximum Likelihood analyses produced highly similar tree topologies. MP bootstrap values were similarly high at nodes well-supported by Bayesian analysis.

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Figure 2 Results of Bayesian phylogenetic analysis of 16s rRNA gene sequences from other phylogenetic groups of bacteria found in PCR-amplified clone libraries from swabs samples of toys and surfaces. Cloned sequences are indicated by "CCTR" (Children's Center Toddler Room) or "CCIR" (Children's Center Infant Room) prefixes followed by the date of sampling and whether the sequence was obtained from a toy (T) or a surface (S). GenBank accession numbers are presented next to the names. The tree also includes sequences from some of the cultured isolates in Table 1. The values above the branches indicate the Bayesian posterior probabilities (above 0.5) under the specified model of evolution for each node. Maximum Parsimony and Maximum Likelihood analyses produced highly similar tree topologies. MP bootstrap values were similarly high at nodes well-supported by Bayesian analysis.

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Figure 3 Graphical representation of common bacterial-type abundance found in the six clone libraries made from furniture surface swabs based on 16S rRNA gene sequence identification. Pseudomonads and uncultured Oxalobacteraceae were consistently found on all surfaces at high levels. Bacillus species were also common, though at lower abundance. One date in particular, Jan 25th,(Toddler Room 2) had a particularly high concentration of Streptococcus-related sequences.

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