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Figures

- Correlation between development of female flower buds and expression of the CS-ACS2 gene in cucumber plants

	Figure 1 Sex of the flowers of gynoecious (RS-G) and monoecious (RS-M) cucumber plants grown in 2003 (Experiment I). The diagrammatic data show the sex of the flowers on each node of the main stems of seven individuals. Closed and open circles represent female and male flowers, respectively. Lower nodes without either circle represent the vegetative nodes. Both RS-G and RS-M plants produced their first flowers on nodes 3 or 4 on the main stems. Flowers produced by RS-G plants were all female, but RS-M plants produced male flowers at approximately nodes 10–12. (Click image to enlarge)
	Figure 2 Sex of the flowers of gynoecious (RS-G) and monoecious (RS-M) cucumber plants grown in 2004 (Experiment II). The diagrammatic data show the sex of the flowers on each node of the main stems of five individuals. Closed and open circles represent female and male flowers, respectively. Lower nodes without either circle represent the vegetative nodes. The first flower appeared on node 5 in RS-G and on node 3 in RS-M plants. All flowers produced by RS-G plants were female, but RS-M plants produced female flowers on node 6–7, 12–13, 19–20, and 24–25, which were separated by 4–5 nodes with male flowers. (Click image to enlarge)
	Figure 3 Accumulation of CS-ACS2 mRNA in different organs of gynoecious (RS-G) and monoecious (RS-M) cucumber plants. Total RNA was extracted from roots, stems, leaves and shoot apices of plants at the 5-leaf stage and analysed by RT-PCR. (Click image to enlarge)
	Figure 4 Accumulation of CS-ACS2 mRNA in the shoot apices of gynoecious (A) and monoecious (B) cucumber plants. Shoot apices were harvested from 5-leaf stage plants and sectioned longitudinally for in situ hybridization. Am, apical meristem of the main shoot; Le, immature leaf; Sm, stem; Se, sepal; Pe, petal; St, stamen or its primodium; Pi, pistil or its primodium; Ov, ovule. Scale bar represents 1 mm. CS-ACS2 signal (arrowhead) is observed specifically in the pistillate tissue of flower buds. (Click image to enlarge)
	Figure 5 In situ hybridization analysis of CS-ACS2 mRNA accumulation in flower buds from successive nodes on the main stem of gynoecious (RS-G) cucumber plants (Experiment I). Flower buds at different stages of development were harvested from nodes 14 (A) to 3 (L) on the main stem of RS-G plants grown in 2003. Numbers in the parentheses indicate the node position on the main stem. Se, sepal; Pe, petal; St, stamen or its primodium; Pi, pistil or its primodium; Ov, ovary. Scale bars for nodes 14 (A) to 11 (C) and for nodes 10 (D) to 3 (K) represent 200 µm and 500 µm, respectively. (Click image to enlarge)
	Figure 6 n situ hybridization analysis of CS-ACS2 mRNA accumulation in flower buds from successive nodes on the main stem of monoecious (RS-M) cucumber plants (Experiment I). Flower buds at different stages of development were harvested from nodes 13 (A) to 3 (K) on the main stem of RS-M plants grown in 2003. Numbers in the parentheses indicate the node position on the main stem. Se, sepal; Pe, petal; St, stamen or its primodium; Pi, pistil or its primodium; Ov, ovary. Scale bars for nodes 13 (A) to 11 (C) and for nodes 10 (D) to 3 (K) represent 200 µm and 500 µm, respectively. (Click image to enlarge)
	Figure 7 In situ hybridization analysis of CS-ACS2 mRNA accumulation in flower buds from successive nodes on the main stem of gynoecious (RS-G) cucumber plants (Experiment II). Flower buds at different stages of development were harvested from nodes 18 (A) to 5 (N) on the main stem of RS-G plants grown in 2004. Numbers in the parentheses indicate the node position on the main stem. Se, sepal; Pe, petal; St, stamen or its primodium; Pi, pistil or its primodium; Ov, ovary. Scale bars for nodes 18 (A) to 15 (D) and for nodes 14 (F) to 5 (N) represent 200 µm and 500 µm, respectively. (Click image to enlarge)
	Figure 8 In situ hybridization analysis of CS-ACS2 mRNA accumulation in flower buds from successive nodes on the main stem of monoecious (RS-M) cucumber plants (Experiment II). Flower buds at different stages of development were harvested from nodes 17 (A) to 5 (M) on the main stem of RS-M plants grown in 2004. Numbers in the parentheses indicate the node position on the main stem. Se, sepal; Pe, petal; St, stamen or its primodium; Pi, pistil or its primodium; Ov, ovary. Scale bars for nodes 17 (A) to 14 (D) and for nodes 13 (E) to 5 (M) represent 200 µm and 500 µm, respectively. (Click image to enlarge)
	Figure 9 Accumulation of CS-ACS2 mRNA in the developing ovary of cucumber plants. Female flower buds were harvested from node 9 of gynoecious (RS-G) plants at the 5-leaf stage for in situ hybridization (A, B). (A, B) The location of CS-ACS2 mRNA accumulation (arrowhead) in the cross-section of the developing ovary. (C) The cross-section of the ovary at anthesis, in which the arrowhead indicates the region of the placenta. Scale bars in (A), (B), and (C) represent 500 µm, 100 µm, and 1 mm, respectively. (Click image to enlarge)