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In this paper the complete mitochondrial genome sequence of Asian arowana that …


Biology Articles » Genetics » Genomics » The complete mitochondrial genome of a basal teleost, the Asian arowana (Scleropages formosus, Osteoglossidae) » Figures

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- The complete mitochondrial genome of a basal teleost, the Asian arowana (Scleropages formosus, Osteoglossidae)

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Figure 1 The schematic diagram and full sequence of the Asian arowana heavy strand control region shows the presence of two repeats. Panel A: Schematic diagram of Asian arowana mitochondrial heavy strand control region. Labels: TRA – tandem repeat array; TAS – termination-associated sequence; CSB – conserved sequence block; MS – microsatellite. Panel B: The nucleotide sequence. Positions of the TACAT motif, 37-bp tandem repeat, termination associated sequences, conserved sequence blocks and the AT-microsatellite are labeled with bold.

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Figure 2 A conserved motif capable of forming a hairpin is present in the mtDNA of several osteoglossid and eel species. Mfold deduced hairpin structure from a repeat unit within the tandem repeat array located in heavy strand control region. Shaded region is the conserved motif TACAT/ATGTA. Panel A: Hairpin structures of the members of Osteoglossiformes superorder. From left: Asian arowana, silver arowana, butterfly fish and goldeneye. Panel B: Alignment of a repeat unit sequence from tandem repeat array of various Anguilliformes superorder members' mitochondrial heavy strand control region. A hairpin structure of Anguillia australis australis constructed using Mfold.

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Figure 3 Nucleotide usage frequency of three osteoglossids compared to that of the bichir. Frequency of nucleotide usage according to codon position for all protein-coding genes. Order of bars from the left: Asian arowana (An external file that holds a picture, illustration, etc., usually as some form of binary object. The name of referred object is 1471-2164-7-242-i1.gif), silver arowana (An external file that holds a picture, illustration, etc., usually as some form of binary object. The name of referred object is 1471-2164-7-242-i2.gif), butterfly fish (An external file that holds a picture, illustration, etc., usually as some form of binary object. The name of referred object is 1471-2164-7-242-i3.gif) and bichir (An external file that holds a picture, illustration, etc., usually as some form of binary object. The name of referred object is 1471-2164-7-242-i4.gif).

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Figure 4 The GC and AT skew for mitochondrial protein-coding genes in Asian arowana mtDNA. Graphical representation of absolute values is shown. Genes are ordered according to their position in the mitochondrial genome.

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Figure 5 Phylogenetic analysis of osteoglossids and other teleosts by using concatenated mitochondrial protein-coding genes. The data sets consist of a total of 3,675 amino acid positions concatenated from 12 protein sequences for each species. The phylogenetic relationship of Asian arowana with respect to representatives from Actinopterygii and Sarcopterygii taxa using dogfish shark as outgroup was performed by maximum parsimony (MP), maximum likelihood (ML) and Bayesian inferences (BI) methods. Tree topology produced by the different methods was similar. Bootstrap values are in parentheses and in MP/ML/BI order.

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Source: BMC Genomics. 2006; 7: 242.

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