A comparison of three methods used to estimate the stomatal density of …

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• Materials and methods
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- A comparison of three methods for determining the stomatal density of pine needles

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Table 1. The stomatal density of each species measured from a dissecting scope, SEM, and light microscope. Numbers in parentheses are the standard errors.

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Table 2. Stomatal densities of pine needles determined from three methods: scanning electron microscopy (SD_sem), maceration (SD_lm), and dissecting light microscope (SD_ij). SD_lm (cor) are stomatal densities of macerated needles after correcting for geometry and changes in needle width from saturation in H₂O.

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Table 3. Stomatal densities measured on fresh, fully expanded (SD_Fresh) Pinus ponderosa and Pinus taeda needles (n=6). Stomatal densities were measured again on the same needles after they were oven-dried for 48 h at 70 °C (SD_Dry), then remeasured after they were saturated in distilled H₂O for 48 h (SD_Wet). A one way ANOVA was used to analyse differences between fresh and oven-dried needles because leaf area of the oven-dried needles should decrease, resulting in higher stomatal densities. Numbers in parentheses are the standard errors.

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Table 4. Area and width increase of air-dried leaves after saturating in distilled water for 48 h. Broad-leaves were analysed by cutting a 1x1 cm section near the midrib. Conifers were analysed by measuring their widths near the centre of the needle. Abies and Pseudotsuga needles were oven-dried at 70 °C before saturating in distilled H₂O.

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Source: Journal of Experimental Botany, Vol. 52, No. 355, pp. 369-373, February 2001.