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A comparison of three methods used to estimate the stomatal density of …


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- A comparison of three methods for determining the stomatal density of pine needles

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Fig. 1. Cross-section of a three-needle pine fascicle. True abaxial (ABWd) and adaxial (ADWd) surface widths are determined from equations 1 and 2, respectively. Because the r's cancel, both equations simplify to 1.209xW for ABWd, and 1.155xW for ADWd. Dotted line represents angle of view from microscope.

 

figure 1

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Fig. 2. Comparison of Pinus taeda ({circ}) and Pinus ponderosa (•) stomatal densities obtained from a binocular dissecting scope (SDd) with stomatal densities obtained from scanning electron microscopy (SDsem). Dotted line represents 1:1 line, solid line represents regression presented in panel.

figure 2

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Fig. 3. Comparison of Pinus taeda ({circ}) and Pinus ponderosa (•) stomatal densities obtained from a binocular dissecting scope (SDd) with stomatal densities obtained from light microscopy (SDlm). (A) Uncorrected for the changes in needle widths after the air-dried needles were saturated in distilled water during maceration. (B) The comparison of stomatal densities after the changes in needle widths were corrected for. Dotted line represents 1:1 line, solid line represents regressions presented in panels.

figure 3

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Source: Journal of Experimental Botany, Vol. 52, No. 355, pp. 369-373, February 2001.


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