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Biology Articles » Cryobiology » Characteristics of fresh gander semen and its susceptibility to cryopreservation in six generations derived from geese inseminated with frozen-thawed semen

Characteristics of fresh gander semen and its susceptibility to cryopreservation in six generations derived from geese inseminated with frozen-thawed semen

Ewa Łukaszewicz

Institute of Animal Science, Department of Poultry Breeding, Agricultural University of Wrocław, 50-631 Wrocław, Chełmońskiego 38c, Poland,
Phone: +48 71 3205-774; fax: +48 71 3205-776; e-mail:
ewa@gen.ar.wroc.pl

Abstract

The paper summarises seven years experiments designed to determine the effect of continuous insemination with frozen-thawed semen on fresh semen quality and sperm susceptibility to freezing stress in succeeding generations. During course of experiments, semen was collected from 10-12 White Kołuda® ganders at the age of 8-9 months, then subjected to freezing and used after thawing for insemination of 10 geese in order to obtain the subsequent generation of males. Semen was diluted 1:0.5 (v/v) with EK diluent, equilibrated for 15 min at +4°C, mixed with 6% (v/v) of dimethyl-formamide (DMF), frozen to temp. -1400C at a rate 60°C/min and then transferred into LN2 container. Semen samples were thawed prior to insemination in a 60°C water-bath. It is difficult to conclude whether freezing stress affected the fresh semen quality, since average volume of SQF (index comprising ejaculate volume, sperm concentration and percentage of live normal cells) varied between generations from 19.3 to 56.2. Continuous goose reproduction by insemination with frozen-thawed semen resulted in significant increase (P 0.01) in spermatozoa resistance to cryoinjury in every subsequent generation. In the relation to adequate fresh semen the percentage of live morphologically intact spermatozoa which withstood freezing procedure increased from 27.2 in 1st generation to 74.4 in 6th generation.

Keywords: gander semen, cryopreservation, spermatozoa morphology

Source: CryoLetters 27 (1), 51-58 (2006).


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