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Biology Articles » Reproductive Biology » Calcium Signals for Egg Activation in Mammals » Figures

Figures
- Calcium Signals for Egg Activation in Mammals

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Fig. 1. Schematic illustration of sperm-egg signaling that leads to successive Ca2+ release from the ER through the IP3R and form

figure 1

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 Fig. 2. Ca2+ oscillations in mouse eggs induced by a spermatozoon at fertilization (A), injection of hamster sperm extract (B), and injection of recombinant PLC. (60 µg/ml) (C). The ordinate is the ratio of fluorescence intensity of fura-2 in the egg activated by 340 and 380 nm lights (F340/F380), reflecting [Ca2+]i.s a Ca2+ wave in mammalian eggs.sword,

figure 2

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 Fig. 3. A: The domain feature of PLC. molecule. B: Ca2+ oscillations after injection of RNA (20 µg/ml) encoding PLC.-Venus. C: Distribution of expressed PLC.-Venus and translocation into the PN. Changes in the fluorescence intensity (arbitrary unit) of Venus in the cytoplasm and pronucleus are presented in 3 eggs. Inset is the bright field image and fluorescence image of a 1-cell embryo at 7.5 h after RNA injection and shows accumulation of expressed PLC. in the PN formed at 4 h. The arrow in the bright field image indicates the PN..

  figure 3

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Fig. 4. Possible signaling pathway downstream the [Ca2+]i rise at fertilization leading to release from M II arrest, formation of the pronuclei, and 1st cleavage of mammalian embryos. The PN of 1-cell embryo and the nuclei of 2-cell embryo are shaded to illustrate the accumulation of the sperm factor or PLC..

 

figure 4

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