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GD3Bu-specific antibodies became potent cytotoxic reagents against Bu-SK-MEL-28 cells when GD3…


Biology Articles » Bioengineering » Bioengineering of Surface GD3 Ganglioside for Immunotargeting Human Melanoma Cells » Footnotes

Footnotes
- Bioengineering of Surface GD3 Ganglioside for Immunotargeting Human Melanoma Cells

* This is National Research Council of Canada Publication Number 42490. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. 

{ddagger} Both authors contributed equally to this work. 

§ To whom correspondence should be addressed. Tel.: 613-990-0821; Fax: 613-941-1327; E-mail: Harry.Jennings@nrc.ca .

1 The abbreviations used are: KLH, keyhole limpet hemocyanin; GD3, {alpha}NeuAc(2->8){alpha}NeuAc(2->3){beta}Gal(1->4){beta}Glc-Cer; ManNAc(Pr, Bu, Bz), N-acetyl(propionyl, butyryl, benzoyl)mannosamine; GD3Pr, GD3Bu, and GD3Bz are modified GD3 in which both N-Ac groups are replaced by N-Pr, N-Bu, and N-Bz groups, respectively; mAb, monoclonal antibody; PSA, polysialic acid; Bu(Ac, Pr, Bz)-SK-MEL-28, SK-MEL-28 cells treated with ManNBu (Ac, Pr, Bz) precursor; BSA, bovine serum albumin; CDC, complement dependent cytotoxicity; PBS, phosphate-buffered saline; ELISA, enzyme-linked immunosorbent assay; MS, mass spectroscopy. 

ACKNOWLEDGMENTS

We thank Dr. Jianjun Li and Don Krajcarsky for MS analysis and Marie-France Karwaski for the purification of enzymes.



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