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In this study, the entrapment of amyloglucosidase from Aspergillus niger into dipalmitoylphosphatidylcholine …


Biology Articles » Bioengineering » Amyloglucosidase enzymatic reactivity inside lipid vesicles » Tables

Tables
- Amyloglucosidase enzymatic reactivity inside lipid vesicles

Table 1

AMG entrapment in DPPC MLVs

DPPC concentration (mg/ml)
Entrapment percent (%)
Entrapment Efficiency (mg protein/mg DPPC)
Activity of entrapped AMG (Unit/mg protein)
2
6.83
0.507
8.86
4
9.35
0.349
5.76
7.8
13.17
0.252
5.84
11.25
14.45
0.188
6.68

Li et al. Journal of Biological Engineering 2007 1:4   doi:10.1186/1754-1611-1-4

Table 2

Kinetic parameters for single and dual enzyme systems.


Vmax
(mg mL-1min-1)
Km
(mg mL-1)
Ki
(mg mL-1)
Free enzyme (intrinsic)
1.28 ± 0.06
1.55 ± 0.29
0.10 ± 0.01
MLV (apparent)
0.35 ± 0.01
1.15 ± 0.12
0.10 ± 0.01
LUV (apparent)
0.56 ± 0.04
1.64 ± 0.25
0.10 ± 0.01

Li et al. Journal of Biological Engineering 2007 1:4   doi:10.1186/1754-1611-1-4


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