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In this study, the entrapment of amyloglucosidase from Aspergillus niger into dipalmitoylphosphatidylcholine …


Biology Articles » Bioengineering » Amyloglucosidase enzymatic reactivity inside lipid vesicles » Figures

Figures
- Amyloglucosidase enzymatic reactivity inside lipid vesicles

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Figure 1.Schematic enzymatic hydrolysis inside a DPPC vesicle.

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Figure 2.Representative micrograph of DPPC MLVs. A: Negative-staining image. B: Freeze-fracture TEM. C: Cryo-SEM image.

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Figure 3.Representative micrograph of DPPC MLVs by confocal microscopy. The unit of the scale bar is μm.

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Figure 4.The entrapment percentage and efficiency as a function of AMG concentration. Filled circle (red circle): Entrapment percent. Filled square (blue square): Entrapment efficiency.

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Figure 5.Thermostability of AMG. Filled circle (red circle): Soluble AMG. Filled square (blue square): MLVs. Filled triangle (green triangle): GUVs. Experiments were carried out at pH 5.0 and temperature 50°C. The initial activities were taken as 100%.

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Figure 6.Initial rate study for entrapped AMG in DPPC. Filled circle (red circle): Soluble AMG. Filled square (blue square): LUVs. Filled triangle (green triangle): MLVs.

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Figure 7.Model simulation and experimental validation. Red (soluble AMG) and blue (MLV) lines are simulation results of glucose concentration profiles. Symbols are experimental results of the glucose concentration profiles: Soluble AMG (filled red circle) and MLV (filled blue square). The green line is a simulation result of starch concentration profile inside MLV.

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Figure 8.Repeated runs using MLVs for enzymatic hydrolysis of starch.

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